Table 4 TF targets of miRNAs identified by degradome sequencing
miRNA FamilyTarget GeneTF Family
miR156MsUN014236SBP
MsUN046666SBP
MsUN050762SBP
MsUN011072SBP
MsUN049469SBP
MsUN086910SBP
miR160MsUN041965ARF
MsUN097717ARF
miR164MsUN043080NAC
MsUN045895NAC
miR167MsUN007721AUX/IAA
miR169MsUN004554CCAAT
MsUN014711CCAAT
MsUN030848CCAAT
MsUN044017CCAAT
MsUN096784CCAAT
MsUN102122CCAAT
miR396MsUN007041GRF
MsUN088040GRF
MsUN104762C3HC4
MsUN104763C3HC4
MsUN101411MADS-box
miR2645MsUN102295bZIP
miR5205MsUN046011AP2-EREBP
miR5249MsUN104998bHLH
miR530MsUN047511NAC
NmiR0007MsUN030863GRAS
NmiR0028MsUN045647G2-like
  • The assembled alfalfa transcript sequences were submitted to the iTAK software for TF identification using default parameters; 28 TFs from 13 families were identified, and the results of hypergeometric test showed that TF genes were highly enriched among these targets.