Table 2 Frequency of ATG in combined coding sequence (CDS), 5UT sequence (5UT), and expected random frequency with length-matched sequence sets with the same dinucleotide frequency as in genomic CDS or 5UT
SequenceATG_inframeATG_outframe
CDS0.0170.011
Rand_CDS0.0100.022
5UT0.0100.019
Rand_5UT0.0130.026
  • Frequencies are numbers detected divided by total sequence length/3. ATG_inframe: in-frame with CDS (excluding the initiator itself); ATG_outframe: out-of-frame with coding sequence. Randomized results were similar if mononucleotide frequencies were used instead of dinucleotides.