Table 1 Annotated 5′ untranslated sequences for all annotated transcripts were extracted from the reference Chlamydomonas genome and analyzed for potential uORF content
uORF Classes Present
Number of Transcript Models123
6438
769+
821+
6693+
421++
1600++
1514++
1270+++
Sums
4060+
4026+
11077+
  • For schematic of classes see Figure 1. Class 1: ATG in 5UT sequence, in-frame with reference CDS, without intervening stop codon; class 2: ATG in 5UT sequence, out-of-frame with reference CDS, without intervening stop codon; class 3: ATG in 5UT sequence, with stop codon in-frame before the reference CDS. A given transcript can in principle have any number of each class. Sums: total transcripts containing at least one of the indicated class of uORFs.