Table 2 CRISPR injection scheme
Injected FliesDonorchiRNA 1chiRNA 2
vas-Cas9.RFP(−) (/FM7a,Tb)rnCR-EGFPrn-chiRNA-intronrn-chiRNA-inter
vas-Cas9.RFP(−) (/FM7a, Tb)rnCR-FLAGrn-chiRNA-intronrn-chiRNA-inter
vas-Cas9.RFP(−); roe[3]/TM3rnCR-EGFPrn-chiRNA-intronrn-chiRNA-inter
vas-Cas9.RFP(−); roe[3]/TM3rnCR-FLAGrn-chiRNA-intronrn-chiRNA-inter
vas-Cas9.RFP(−); rn-EGFP DsRed(−)rnC-STOPrnC-chiRNA-5′rnC-chiRNA-3′
  • vas-Cas9.RFP(−) chromosome (FBst0055821) is from Bloomington stock #55821; roe[3] chromosome (FBst0007411) is from #7411. The majority of the injected embryos from the first and second experiments are homozygous for vas-Cas9.RFP- (thus parentheses for FM7a); all the rest are homozygous for vas-Cas9.RFP(−).