Table 1 CRISPR oligos
Primer IDNameSequence
1rn-chiRNA-intron-sensecttcGTTGTGAAGAATCGAAGAGA
2rn-chiRNA-intron-antisenseaaacTCTCTTCGATTCTTCACAAC
3rn-chiRNA-inter-sensecttcgATATTCCGAGACACAGGGGA
4rn-chiRNA-inter-antisenseaaacTCCCCTGTGTCTCGGAATATc
55HDR-rnCR-FggtaccCATCAGCGCAACAACCTGG
65HDR-rnCR-RctcgagTCCCTTGTCCTTCCCAGGA
7EGFP-cDNA-NctcgagATGGTGAGCAAGGGCGAG
8EGFP-cDNA-CgtcgacTTACTTGTACAGCTCGTCCATGC
93G4S-Xhol-FtcgagGGAGGAGGCGGCTCCGGAGGCGGAGGATCCGGCGGAGGTGGCTCCc
103G4S-Xhol-RtcgagGGAGCCACCTCCGCCGGATCCTCCGCCTCCGGAGCCGCCTCCTCCc
113G4S-3XFLAG-FtcgagGGAGGAGGCGGCTCCGGAGGCGGAGGATCCGGCGGAGGTGGCTCCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGATTACAAGGATGACGATGACAAGTAAg
123G4S-3XFLAG-RtcgacTTACTTGTCATCGTCATCCTTGTAATCGATGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCGGAGCCACCTCCGCCGGATCCTCCGCCTCCGGAGCCGCCTCCTCCc
133HDR-rnCR-FgcggccgCCTGTGTCTCGGAATATCATTTTGG
143HDR-rnCR-RgagctcATTGCAAGGGGTCTGAACTG
153UTR-rnCR-FgtcgacCTAGGGGCCTACTTCTAGATGG
163UTR-rnCR-RaagcttGGAAGGATAACATTTAATTTACTTTATTACG
17rnC-chiRNA-5′-FcttcGGCGGAATCTCCCCAATCAG
18rnC-chiRNA-5′-RaaacCTGATTGGGGAGATTCCGCC
19rnC-chiRNA-3′-FcttcGATCCGGGACTTGCGGCCCC
20rnC-chiRNA-3′-RaaacGGGGCCGCAAGTCCCGGATC
21rnC-5HDR-Kpnl-FggtaccATGTCTGCGCCTGAATGACT
22rnC-5HDR-Spel-RactagttaattagttaCAGCGGCGAGTTGTGATGGTAG
23rnC-3HDR-Notl-FgcgGCCGCAAGTCCCGGATCTAC
24rnC-3HDR-Sacl-RgagctcGATGCCTGCACTTGTACGG
  • All restriction enzyme sites for cloning are underlined. The nucleotide labeled in red from ID 3 or 4 is the addition of g/c to aid transcription by the U6 promoter. The sequence labeled in red from ID 11 or 12 encodes 3XFLAG. The four triplets labeled in red from ID 22 are the added stop codons.