Table 1 Sampling the Notch opa repeat genotype via Sanger sequencing
Group% Non-opa31aAvg. DeviationbRAL Lines (Notch opa genotype)
Notch Dirty Dozenc58.3% (7/12)1.7 a.a.21 (opa31), 237 (opa35a1), 350 (opa31), 439 (opa31), 440 (opa31), 555 (opa33a), 646 (opa23), 776 (opa33a), 796 (opa33a), 801 (opa32), 802 (opa32), 822 (opa31)
Radiation-resistantd58.3% (10.5/18)1.8 a.a.91 (opa31), 69 (opa32), 338 (opa32), 208 (opa31), 57 (opa33b), 492 (opa31), 142 (opa31/32), 879 (opa31), 370 (opa36e/opa37), 808 (opa31), 237 (opa35a1), 88 (opa37), 405 (opa31), 646 (opa23), 801 (opa32), 105 (opa33b), 776 (opa33a), 195f (opa31)
Radiation Sensitiveg31.8% (7/22)0.6 a.a.21 (opa31), 350 (opa31), 365 (opa31), 439 (opa31), 440 (opa31), 555 (opa33a), 712 (opa31), 796 (opa33a), 802 (opa32), 820 (opa34), 822 (opa31), 832 (opa31), 837 (opa31), 852 (opa35a2), 859 (opa32), 861 (opa32), 882 (opa31), 887 (opa31), 890 (opa31), 897 (opa31), 908 (opa31), 911 (opa31)
In(3R)K/In(3R)Kh100.0% (3/3)3.7 a.a.100 (opa32), 105 (opa33b), 646 (opa23)
  • a Each haplotype in the heterozygous RAL-142 and RAL-370 stocks counted as 0.5.

  • b Average absolute value of deviation from a wild-type length of 31 amino acids (a.a.).

  • c DGRP1.0 lines with ambiguous assembly sequence (contains Ns) or His → Gln (RAL-646).

  • d Top radiation-resistant RAL lines genotyped in order of average survival (Vaisnav et al. 2014): 91 (49.0/50 flies); 69 (47.5/50 flies); 338 (45.0/50 flies); 208 (44.5/50 flies); 57 (43.0/50 flies); 492 (42.0/50 flies); 142 (41.0/50 flies); 879 (39.0/50 flies); 370 (49/50 flies); 808 (31.5/50 flies); 237 (28.5/50 flies); 88 (26.5/50 flies); 405 (26.0/50 flies); 646 (9.5/50 flies); 801 (4.5/50 flies); 105 (2.0/50 flies); 776 (2.0/50 flies); 195 (2.0/50 flies).

  • e The opa36 variant was observed only once in three sequences from a pool of three males and not observed in sequences from three individual males (total of 1/8 sequences).

  • f By virtue of a nonsynonymous substitution, this RAL line uniquely has a Q7 Twist, whereas other lines have a Q2LQ4.

  • g Radiation-sensitive RAL lines genotyped [0/50 flies surviving (Vaisnav et al. 2014)].

  • h Only three RAL lines are homozygous for Kodani inversion (Huang et al. 2014).