Table 1 Synonymous and nonsynonymous variation in D. melanogaster mitochondrial genes and OXPHOS complexes
Gene/ComplexaSynonymous SitesbNonsynonymous Sites
n (bp)SπӨWDn (bp)SπӨWD
COI347161.573.81−1.911183000Undef
COII14250.311.19−1.9053910.050.24−1.13
COIII169.3390.722.14−1.96613.6710.050.24−1.13
ND119430.160.71−1.7273930.160.71−1.72
ND219850.411.19−1.6982260.341.43−2.07
ND36810.050.24−1.1228010.050.24−1.12
ND4273.3370.511.67−1.951061.6770.381.67−2.17
ND4L5610.050.24−1.1322920.110.48−1.49
ND5351100.682.38−2.16136540.210.95−1.88
ND6103.3340.260.95−1.75415.6720.110.48−1.49
Complex I1243.66312.127.38−2.484912.34251.355.95−2.64
Complex III23950.551.19−1.3889220.160.48−1.29
Complex IV658.33302.607.14−2.212335.6720.110.48−1.49
Complex V174.6720.160.71−1.72650.3360.321.43−2.10
Total2315.66685.4316.42−2.448790.34351.938.33−2.70
  • OXPHOS, oxidative phosphorylation; Undef, undefined; CO, cytochrome c oxidase; ND, NADH dehydrogenase; ATPase, ATP synthase.

  • a Complex I (ND), 7 loci; Complex III (Cytochrome B), 1 locus; Complex IV (CO), 3 loci; Complex V (ATPase), 2 overlapping loci.

  • b For synonymous and nonsynonymous sites, we calculated the number of segregating sites (S), heterozygosity (π), Watterson’s ӨW, and Tajima’s D. The heterogeneity test for differences between synonymous and nonsynonymous D was never significant (P > 0.35 for all comparisons).