Table 1 Possible scenario after bombardment of leaf material
ScenarioFluorescence
(G1) nontransformed cells (not met by a DNA-coated gold particle)Green
(G2) transformed cells where TALENs did not create a DSB in the target site
(G3) cells with both gfp alleles being unaltered owing to correct repair or with a mutation not affecting gene function
(G4) cells with one gfp allele being functionally affected via NHEJ, whereas the other one is unaltered or its function is retained despite of a (minor) mutation
(GY1) cells with one gfp allele being modified by HDR using the yfp*-repair template, whereas the other one remained functionally unaffectedGreen and yellow
(Y1) cells with both gfp alleles being modified by HDR using the yfp*-repair templateYellow
(Y2) cells with one gfp allele being modified by HDR using the yfp*-repair template, whereas the other one is functionally affected by NHEJ
(N1) cells with both gfp alleles being functionally affected by NHEJNo
(N2) cells that have not survived
  • TALENs, transcription activator-like effector nucleases; DSB, double-strand break; NHEJ, nonhomologous end joining; HDR, homology-directed repair; yfp*, truncated yellow fluorescent protein.