Table 1 Transgenic cas9 lines used in this study

Construct(s)	Name (s)	Cas9 Coding Sequence^a (Addgene Plasmid Number)	Integration Site of cas9 Construct (Chromosome Arm)	Reference
act-cas9^b	CFD1; BL54590	Hs_Cas9 (41815, 62209)	attP-ZH2a (X)	Port et al. (2014)
vasa-cas9	BL56552	Hs_Cas9 (42230)	attP-VK00037 (2L)	Gratz et al. (2014)
vasa-cas9^b	BL51324	Hs_Cas9 (42230)	attP-VK00027 (3R)	Gratz et al. (2014)
vasa-cas9^b	BL51323	Hs_Cas9 (42230)	attP-ZH2a (X)	Gratz et al. (2014)
vasa-cas9	BL52669	Hs_Cas9 (41815)	attP-ZH2a (X)	Sebo et al. (2013)
nos-cas9^b	CFD2; BL54591	Hs_Cas9 (41815, 62208)	attP-ZH2a (X)	Port et al. (2014)
nosG4VP16 UAS-cas9^b	CFD3_nos; BL54593	Dm_Cas9	attP2 (3L)	Port et al. (2014)
nos-cas9:GFP^b	CFD8	Hs_Cas9:GFP (42234)	attP-ZH2a (X)	Port et al. (2014)
nos-cas9	TH00787.N	Dr_Cas9	attP2 (3L)	Ren et al. (2013)
nos-cas9	TH00788.N	Dr_Cas9	attP40 (2L)	Ren et al. (2013)
nos-cas9	CAS-0001	Hs_Cas9 (41815)	attP40 (2L)	Kondo and Ueda (2013)
nos-cas9	CAS-0002	Hs_Cas9 (41815)	Random insertion (X)	Kondo and Ueda (2013)
nos-cas9	CAS-0003	Hs_Cas9 (41815)	Random insertion (3)	Kondo and Ueda (2013)
nos-cas9	CAS-0004	Hs_Cas9 (41815)	Random insertion (2 (CyO balancer))	Kondo and Ueda (2013)

act, actin5C; nos, nanos; BL, Bloomington Drosophila Stock Center stock number; Hs, Homo sapiens; Dm, Drosophila melanogaster; Dr, Danio rerio.
↵a Species refers to codon optimization; all constructs express Streptococcus pyogenes Cas9 protein.
↵b Lines that were also evaluated in Port et al. (2014).