Table 2 DnaQ insertion mutants
Amino Acid PositionInsertion Composition5-bp Target Sequence
30IICLNTCATT
57LVFKQGCTGG
76CLNNKATAAG
79CLNTFCGTTT
97ILFKQGATCC
115KLFKHTAAGC
125LFKHFTTTCT
132V*CTTGC
143NSCLNGTTGA
162LFKQLGCTGC
196LFKQQGCAAC
208VFKHQCCTGA
224AHV*GCTCA
228CLNKAAAGCC
232LFKQVGGTGC
  • The final 15-bp insertion duplicates a 5-bp target and adds a 10-bp sequence (TGTTTAAACA) containing restriction site PmeI