Table 1 KASP assay screening results for G. barbadense–derived markers
TypeMarker NameKASP ResultIdentified in G. barbadense MappingHomeo-SNP RemovalFinal Result
Iteration 1Iteration 2Iteration 1Iteration 2Iteration 1Iteration 2
Not Transcriptome-AssociatedGH_TBb001A07f_381GoodYesYesRetainRetainGoodGood
GH_TBb001A07f_486BadNoYesRemove
GH_TBb001A23r_531BadNoYesRetainBad
GH_TBb001A23r_614GoodYesYesRetainRetainGoodGood
GH_TBb001D22r_204GoodYesYesRetainRetainGoodGood
GH_TBb001D22r_445BadYesNoRetainBad
GH_TBb001D22r_511BadYesYesRetainRetainBadBad
GH_TBb001B05f_180GoodYesYesRemoveRemove
GH_TBb001B05f_564BadYesYesRemoveRemove
GH_TBb001C03f_116GoodNoYesRetainGood
GH_TBb001C03f_401GoodYesYesRetainRetainGoodGood
GH_TBb001F01r_117BadYesYesRemoveRemove
GH_TBb001F01r_310BadYesNoRemove
GH_TBb001A17r_218BadYesYesRemoveRemove
GH_TBb001F06f_303BadNoYesRemove
Transcriptome-AssociatedGH_TBb004J20r_76GoodYesYesRemoveRemove
GH_TBb004J20r_348GoodYesYesRetainRetainGoodGood
GH_TBb053N14f_270GoodYesYesRetainRetainGoodGood
Gh_TBh036B20r_583GoodNoYesRetainGood
GH_TBr162H20f_547BadYesYesRemoveRemove
GH_TBb046O02r_64BadYesYesRemoveRemove
GH_TBb046O02r_138BadYesYesRemoveRemove
GH_TBb046O02r_418BadYesYesRemoveRemove
GH_TBb069A06f_248BadYesNoRetainBad
GH_TBb069A06f_304BadYesYesRemoveRemove
GH_TBh034D07r_276GoodYesYesRetainRetainGoodGood
GH_TBh030P12r_332BadYesYesRemoveRemove
GH_TBb119O19f_465Maybe (DOM)YesYesRetainRetainN/AN/A
GH_TBh055K17f_57BadYesYesRemoveRemove
GH_TBh055K17f_506BadYesYesRemoveRemove
GH_TBh023O21f_162GoodYesNoRemoveRetainGood
GH_TBh023O21f_537GoodYesYesRetainRetainGoodGood
  • KASP assay screening results for G. barbadense–derived markers for mapping parameter optimization in CLC Genomics Workbench. Iteration-1 was performed using the following mapping parameters: 0.70 length fraction and 0.99 similarity fraction. Iteration-2 was performed using the following mapping parameters: 0.99 length fraction and 0.98 similarity fraction.