Table 1 Primers used for pyrosequencing
GeneForward PrimerReverse PrimerSequencing PrimerB6CastSNP PositionPCR
Igf2rCCAGAGGACACACAGCTGAAaCACTGTGACCCTCTGGTGAACCCAGAGTTCAGCCACGAGAGAchr17: 1287954128×
Igf2GGGTGTCAATTGGGTTGTTTAGGGACAGTTCCATCACGTCaAATCAAATTTGGTTTTTTAGAACTchr7: 14983951928×
H19CCGAGACGATGTCTCCTTTGGGTATAGCTGGCAGCAGTGGaGAGGCCAGCCGCTTCTTCTCTchr7: 14976359728×
AK017220CCTGATGACTGCAAACATGGaCCAGCTTAGCCAACCTGAAGGGTGGCTTCTGAAATCCTGCGchr11: 5100353732×
Tsci6TCAGGAAAGTGAAGCGAGGTaAAGGCAGTGATGGCAGAGATGGCCATGCATATCTCTTGCAGchr15: 9699052134×
Gab1GGGGTCATTGGCACATAGTTGAGGTGTCTCGGGTGAAGAGaAGCTGCCGGAATGTTGTCGACchr8: 8330874328×
QkCTCTGCCATGTCCTGGACTCACTAAAAACAAACCCACTTCCAaCAGTACACACAGGTAATGTTCchr17: 1040418930×
Dact2ACACCCTTCTGAATCGTTGCaCAGAGTAGGCCCACACTTGCCCACCATTGCCGAGACCAGGAchr17: 1433297034×
  • Single nucleotide polymorphism (SNP) positions are relative to mm9 genome build. Polymerase chain reaction (PCR), the number of PCR cycles used to amplify target genes in each assay.

  • a Location of biotin moiety.