Table 3 Confirmation of CO positions in wt and recq4a populations
IDPop.Plant IDChr.Pos., bpUpaDownaFragment SizebNcFirst MarkerdLast MarkerdConfirmed?
1wt1254134,048Col-0Het9662133,527134,493Yes
2wt145416,276,940HetCol-01567316,275,69816,277,265Yes
3wt147129,632,761Col-0Het1448329,532,39529,533,843Yes
4wt125410,419,728HetCol-0675410,419,34710,420,022Yes
5wt139524,954,623HetWs-2784524,954,06924,954,853No (Ws-2)
6recq4a23134,957,859HetWs-2111864,957,3224,958,440Yes
7recq4a253526,618,925HetCol-01351226,568,24926,569,600No (Het)
8recq4a261212,206,209Ws-2Het779312,206,20912,206,988No (Het)
9recq4a27811,850,178HetWs-2121061,849,6921,850,902Yes
10wt3083172,226Col-0Het12427171,475172,717Yes
11wt387310,014,511Col-0Het1012210,013,64110,014,653Yes
  • CO, crossover; wt, wild type; Pop., population; Chr., chromosome; Pos., position; PCR, polymerase chain reaction.

  • a Genotypes predicted up or downstream of the CO point.

  • b Size of the PCR fragment amplified.

  • c Number of markers covered by Sanger sequencing reads.

  • d Position of the first and last markers covered by the Sanger sequencing reads (in bp)