Table 3 Larval gene expression changes in response to ethanol
GeneExpression ChangeProtein FunctionPredicted Effect
Oxidative stress-relatedCyp4e3121-fold increaseCytochrome P450Increased ROS
Cyp6a24.2-fold increaseCytochrome P450Increased ROS
CG32750, CG32751, vanin-like3.6- to 12.5-fold increasesPantetheine hydrolasesIncreased sensitivity to ROS/increased fatty acid synthesis
Cyp4d216.7-fold decreaseCytochrome P450
GSTD84.6-fold decreaseGlutathione-S-transferaseIncreased ROS
GSTD44.3-fold decreaseGlutathione-S-transferaseIncreased ROS
Lipid metabolism
Gpdh4.1-fold increaseGlycerol-3-phosphate dehydrogenaseFatty acid accumulation
GlcT-14-fold increaseCeramide glucosyltransferaseFatty acid accumulation
Acox57D-d3.9-fold increaseAcyl-coenzyme A oxidaseFatty acid accumulation
Gpo-13.2-fold increaseMitochondrial glycerol-3-phosphate dehydrogenaseFatty acid accumulation
CG17191, CG17192, CG6271, CG6277, CG6283, CG6295, CG80935.1- to 216-fold decreasesTriglyceride lipasesFatty acid accumulation
CG31148, CG31414, CG330906.4- to 11-fold decreasesGlucosylceramidasesFatty acid accumulation
aSMase9.9-fold decreaseAcid SphingomyelinaseReduced ceramide
ATPCL8.3-fold decreaseATP citrate lyasePossible increased storage of triglycerides
  • RNA was isolated from third instar wandering larvae reared in either 0% or 7% ethanol, and gene expression was compared using Affymetrix Drosophila 2.0 microarray chips. Data are presented as fold-change in 7% reared larvae relative to control larvae (developing in 0% ethanol).