Table 2 The 44 identified suppressors in the WEE-1.3 RNAi screen
GeneAverage Brood SizeaAverage Hatching %aPredicted Function
sago-2b153.995.50Argonaute homolog partially required for the amplification phase of RNAi responses
cdk-7136.26.66Cyclin-dependent kinase
cdk-1132.81.69Cyclin-dependent kinase
snr-186.511.60Ortholog of human small nuclear ribonucleoprotein SmD3, biogenesis and function of snRNPs
Y49E10.2165.738.21Novel protein of unknown function
B0336.764.830.16Protein with a THAP or THAP-like domain
rpn-951.63.85Non-ATPase subunit of the 19S regulatory complex of the proteasome
rpn-832.52.3226S proteasome regulatory complex, subunit RPN8/PSMD7
unc-9732.433.56LIM domain-containing protein of the PINCH family, highly similar to human LIMS1 and LIMS2
attf-230.227.29AT hook Transcription Factor family
R10H10.326.931.13C-type lectin
msh-625.822.71Mismatch repair ATPase MSH6
rpt-525.47.92Triple A ATPase subunit of 26S proteasome’s 19S regulatory particle (RP) base subcomplex
bir-222.730.81Protein with two BIR domains that may be involved in apoptosis
rpn-221.44.65Non-ATPase subunit of the 26S proteasome’s 19S regulatory particle (RP) base subcomplex
etr-121.319.15Muscle-specific ELAV-type RNA-binding protein
rpn-6.119.90.75Non-ATPase subunit of the 19S regulatory complex of the proteasome
F36A2.718.527.28Novel protein of unknown function
acdh-1217.817.34Orthologous to the human gene ACYL-CoA DEHYDROGENASE, VERY LONG-CHAIN
acr-2317.225.38Subunit of the nicotinic acetylcholine receptor which encode ligand-gated ion channels
F11A3.215.631.03Translation initiation factor, guanine nucleotide exchange factor
C18E3.915.416.80Novel protein of unknown function
cul-215.06.02E3 ubiquitin ligase
prx-514.315.18Ortholog of the human receptor for type I peroxisomal targeting signal protein, PXR1 (or PEX5)
mop-25.312.324.28Divergent ortholog of fission yeast Mo25p and budding yeast Hym1p
C47E8.412.321.08Conserved protein that is a member of the FAM50A/XAP5 family of proteins
K04H4.211.925.69Secreted protein with an N-terminal chitin-binding peritrophin-A domain
C14B9.211.311.77Protein disulfide isomerase (prolyl 4-hydroxylase beta subunit)
unc-1111.125.70Clathrin-adaptor protein that functions in clathrin-mediated endocytosis
taf-110.49.75Transcription initiation factor
B0280.910.313.91Novel protein of unknown function
sel-810.218.25Nuclear protein required for GLP-1 and LIN-12 signaling
imb-210.111.81Importin beta family protein
grk-19.123.40Serine/threonine protein kinase, most closely related to G protein-coupled receptor kinases
inx-148.610.10Member of the innexin family
F42H10.57.915.39Novel protein of unknown function
cogc-26.811.37Ortholog of mammalian COG-2, a subunit of lobe A of the conserved oligomeric Golgi complex
sex-16.416.52DNA-binding protein, nuclear hormone receptor superfamily of transcriptional regulators
F02A9.45.825.10Orthologous to human SIMILAR TO METHYLCROTONOYL-COENZYME A CARBOXYLASE 2 (BETA)
henn-15.730.06HEN1 ortholog, RNA 3′ end methyltransferase
sdz-244.813.17SKN-1 Dependent Zygotic transcript, single-stranded DNA-binding replication protein A
glr-64.59.62Putative non-NMDA ionotropic glutamate receptor subunit
mrps-304.47.88Mitochondrial 28S ribosomal protein S30
C36E8.14.216.55Novel protein of unknown function
nud-13.311.49Nuclear distribution protein
  • a reported averages for animals co-depleted of WEE-1.3 and the indicated candidate gene

  • b sago-2 is a global RNAi suppressor that acts by reducing RNAi efficacy and therefore is not considered a true suppressor.