Table 1 Summary of the transformation efficiencies for the four different genomic locations modified in this study
LocationInjected GenotypesgRNAsInjected EmbryosLarvaeG0 AdultsFertile G0 AdultsIndependent G0 FoundersTotal Red-Eyed F1Independent Founders Per Fertile G0s
salm[1st exon-dsRed]Lig4−/−sgRNA1 + sgRNA31500396166ND00ND
salm[1st intron-dsRed]Lig4−/−sgRNA3700171149ND211ND
salm[3rd exon-dsRed]Lig4−/−sgRNA7 + sgRNA91500312150ND472ND
salm[1st exon-dsRed]Act5C-Cas9, Lig4−/−sgRNA1 + sgRNA370012448301133.3%
salm[1st intron-dsRed]Act5C-Cas9, Lig4−/−sgRNA3 + sgRNA47002001226495414.1%
salm[3rd exon-dsRed]Act5C-Cas9, Lig4−/−sgRNA7 + sgRNA9700291150994594.0%
bentAct5C-Cas9, Lig4−/−bent -sg1 + bent -sg370020411856151.8%