Table 2 Most spn-E mutant females lay eggs with dorsal/ventral patterning defects
Allele Name% Wild-Type% Fused% None% CollapsedTotal Eggs
spn-E/Bal100000600+
Alleles with mutation in DExH box
2A9-1400.48 ± 0.0785.9 ± 3.593.7 ± 3.6428
7G2-54.3 ± 3.33.9 ± 1.93.4 ± 2.688.5 ± 7.8759
155-555.7 ± 2.412.1 ± 2.518.5 ± 9.363.7 ± 9.5742
23-1754.7 ± 9.131.5 ± 2.110.7 ± 2.54.2 ± 3.3970
8D4-11000.95 ±1.399.1 ± 1.3181
Alleles with mutation outside of predicted domains
9A2-1759.4 ± 23.537.9 ± 21.62.6 ± 1.70.15 ± 0.21771
4-4868.4 ± 12.828.9 ± 13.12.4 ± 0.0420.31 ± 0.016750
Allele with mutation in Zn finger
66-2192.1 ± 1.97.2 ± 1.80.56 ± 0.330.16 ± 0.231129
Alleles with premature stop codons
100-371.8 ± 2.011.5 ± 3.828.2 ± 4.658.6 ± 10.3630
114-330.24 ± 0.344.3 ± 0.9912.1 ± 7.683.4 ± 8.3677
9A9-18000100410
4E2-140.36 ± 0.52.3 ± 2.26.25 ± 2.891.1 ± 0.07414
6160.1 ± 0.1431.5 ± 7.820 ± 1.448 ± 9.9687
6534 ± 0.014 ± 4.29.5± 3.577 ± 14.1414
  • Dorsal-ventral patterning defects were quantitated by collecting eggs from spn-Emutant/spn-E∆125 female flies and determining the percentage of eggs with two dorsal appendages (wild-type), fused dorsal appendages, no dorsal appendages, and eggs that were collapsed. Calculations are from two separate experiments, each consisting of three 24-hr egg collections, with the exception of 653 for which the data were from two separate experiments, each consisting of two 24-hr egg collections.