Table 1 Overview of the spn-E mutant phenotypes
Allele NameSPN-E ProteinMutationAffected DomainD/V DefectsRetrotransposon ExpressionDynein Aggregate FormationAub Nuage Localization
2A9-14+Thr145IleDExH box (I)Severe↑↑↑YesDelocalized
7G2-5+Thr233IleDExH box (I/II)Severe↑↑↑YesDelocalized
155-55+Glu239LysDExH box (II)Mid↑↑↑YesDelocalized
23-17+His241GlnDExH box (II)MildNoPartial
8D4-11+Ser435PheDExH box (V)Severe↑↑↑YesDelocalized
9A2-17+Pro508LeuNoneMild↑↑NoPartial
4-48+Glu717LysNoneMildNoNuage
66-21+His1422LeuZn fingerMildNoNuage
100-37G→A bp 488 splice siteTruncated at aa 151Mid↑↑↑YesDelocalized
114-33Arg918stopTruncated at aa 918Severe↑↑↑YesDelocalized
9A9-18Trp632stopTruncated at aa 632Severe↑↑↑YesDelocalized
4E2-14Arg695stopTruncated at aa 695Severe↑↑↑YesDelocalized
616Arg1081stopTruncated at aa 1081Mid↑↑↑YesDelocalized
653Tyr636stopTruncated at aa 636Severe↑↑↑YesDelocalized
  • + or − for SPN-E protein indicates: (+) SPN-E protein detected; (−) SPN-E protein not detected on Western blot analysis. Number in parentheses next to DExH box indicates the DExH box motif affected. Severe D/V defects indicate mostly collapsed eggs laid by mutant females; mid indicates the majority of eggs laid were collapsed; however, a small percent have wild-type or fused dorsal appendages; mild indicates the majority of eggs laid were wild-type. ↑↑↑ indicates all retrotransposons were upregulated in the mutant ovaries; ↑↑ indicates that at least two retrotransposons tested were upregulated; ↑ indicates that one or no retrotransposons was upregulated in the mutant ovaries. bp numbering for 100-37 according to Ensemble Genome Browser. ND, not determined.