Table 3 Results of mutagenesis through HDR
MethodEmbryosG0 AdultHDR Rate, % (n)
TotalSurvival Rate, %*FertileFertile Rate, %**HDR-Yielding G0/Fertile G0HDR-Positive G1/Total G1
Cas950714342.966.7 (2/3)30.5 (111/364)
Cas9D10A54814.8810012.5 (1/8)0.29 (3/1021)
  • * The survival rate is calculated as the percentage of total G0 adults divided by embryos injected.

  • ** The fertile rate is calculated as the percentage of fertile G0 flies divided by total G0 adults.