Table 1 Biofilm gene GO processes for 71 genes that were essential for biofilm development and 100 genes that induced biofilm when deleted (all gene names are given in Table S3)

Mutants Making Significantly Less Biofilm		Mutants Making Significantly More Biofilm
GO Process Annotation	No. of Mutants	GO Process Annotation	No. of Mutants
Biological process unknown	19	Mitochondrion organization	38
Transcription from RNA polymerase promoter	12	Mitochondrial translation	23
Protein targeting	11	Protein complex biogenesis	13
Response to chemical	7	Cellular respiration	10
Invasive growth in response to glucose limitation	5	Biological process unknown	10
Pseudohyphal growth	5	Cell wall organization or biogenesis	8
Carbohydrate metabolic process	5	Cellular amino acid metabolic process	8
Protein complex biogenesis	5	Sporulation	6
Regulation of transport	4	Transmembrane transport	5
Nucleobase-containing small molecule metabolic process	4	Carbohydrate metabolic process	5
Transmembrane transport	4	DNA replication	4
Mitochondrion organization	4	Mitotic cell cycle	4
Endosomal transport	3	Monocarboxylic acid metabolic process	3
Sporulation	3	Cofactor metabolic process	3
Cell wall organization or biogenesis	3	RNA splicing	3
Signaling	3	Oligosaccharide metabolic process	2
Membrane invagination	2	Membrane invagination	2
Protein maturation	2	Protein glycosylation	2
Organelle fusion	2	Telomere organization	2
Cellular amino acid metabolic process	2	Cytokinesis	2
Lipid metabolic process	2	Cytoplasmic translation	2
Amino acid transport	1	Ion transport	2
Protein folding	1	DNA-templated transcription, initiation	1
Ribosomal large subunit biogenesis	1	Vesicle organization	1
Cellular ion homeostasis	1	Cellular ion homeostasis	1
Cytoplasmic translation	1	Meiotic cell cycle	1
Protein farnesylation	1	Golgi vesicle transport	1
		Nucleobase-containing small molecule metabolic process	1

If two GOs capture identical genes, then only one GO is mentioned (lowest p-value). http://www.yeastgenome.org/cgi-bin/GO/goTermFinder.pl.