Table 2 Pattern of nucleotide variation at age-1 and at its immediate upstream and downstream neighbors in three populations of C. remanei
SampleLocusNn% SeqNSPASππaπsπsiDTajHFW
OhioCRE02131224410018606632310.660.7629.2419.971.069−1.208
srh-442550100190533282.460.205.834.03−1.150−3.924**
CRE0212924241009618111.730.161.403.58−0.498−0.536
age-124481003961399251.090.442.572.56−1.821*−2.604*
mdt-81717100244857376.941.448.529.51−0.024−0.041
CRE01736224410050911438.775.1915.2314.892.004−1.492
CRE01735193898337170238.052.5013.808.822.2360.354
GermanyCRE0213122441001869683208.510.7417.9015.760.073−1.804*
srh-4423461001916541135.620.309.959.28−0.440−0.882
CRE02129244810096014331.500.391.742.82−1.470−0.347
age-1244810039597118383.001.238.247.08−0.910−2.293*
mdt-82346681682559125.151.779.157.43−1.067−2.244*
CRE017362550100510138116.014.639.678.340.9991.052
CRE01735193899339310811118.725.1427.299.250.681−0.201
OntarioCRE02131193810018695151710.161.6723.7118.082.167−0.515
srh-441734100192240085.82010.329.790.516−0.926
CRE0212921421009605211.540.723.082.501.2330.625
age-12040100396119891.120.792.211.88−0.376−0.423
mdt-8173473180233653.621.464.595.02−0.586−2.262*
CRE01736193810051013458.576.5014.6612.091.0391.092
CRE01735173498337487456.922.7314.797.520.355−2.233*
PooledCRE0213163126100185510183010.621.2025.9819.540.237−1.062
srh-44651301001901832174.590.199.117.65−1.350−2.287*
CRE021296913810096022641.880.432.523.59−1.233−0.873
age-16813610039599423531.890.874.734.24−1.839**−2.897*
mdt-8571146816827613156.821.959.5110.35−0.472−1.582
CRE017366613210050920959.086.3315.2913.771.3670.716
CRE01735551109733301486118.212.6620.088.96−0.076−1.078
  • Tajima’s D was computed using silent sites diversity. π values are ×103. N, number of strains; n, number of chromosomes, twice the number of the strains when heterozygote sites were present; % Seq, percent of the C. remanei gene sequenced; NS, number of sites analyzed (excluding gap positions); P, number of polymorphic sites; A, number of amino acid replacement changes; S, number of synonymous changes; π, total nucleotide diversity; πa, nucleotide diversity at nonsynonymous sites; πs, nucleotide diversity at synonymous sites; πsi, nucleotide diversity at silent sites.

  • * P < 0.05.

  • ** P < 0.01.