Table 2 Genes identified in the sporulation-defective screen
GeneGene IDCommentsa
Class 1. Genes required for zygote formation (n = 7)
 atg12+SPAC1783.06cAutophagy-associated ubiquitin-like modifier
 cyp9+SPCC553.04Predicted cyclophilin family peptidyl-prolyl cis-trans isomerase
 mmd1+SPAC30C2.02Predicted deoxyhypusine hydroxylase
 prm1+SPAP7G5.03Integral membrane protein important for cell−cell fusion
SPBC1711.12Predicted oxidized protein hydrolase
SPBC18E5.08Predicted N-acetyltransferase
SPBC146.02Sequence orphan
Class 2. Genes required for spores to be iodine-reactive (n = 7)
 fsc1+SPAC22H12.05cFasciclin domain protein
 lcf2+SPBP4H10.11CLong-chain-fatty-acid-CoA ligase
 mam3+SPAP11E10.02cCell agglutination protein
 mcl1+SPAPB1E7.02cDNA polymerase alpha accessory factor
 php3+SPAC23C11.08CCAAT-binding factor complex subunit
 php5+bSPBC3B8.02CCAAT-binding factor complex subunit
 rik1+SPCC11E10.08Silencing protein
Class 3. Genes required for entry into meiosis or for the initiation of forespore membrane assembly (n = 5)
 mei2+SPAC27D7.03cRNA-binding protein required for meiosis
 mei3+SPBC119.04Required for the initiation of meiosis
 mei4+SPBC32H8.11Transcription factor regulating meiotic gene expression
 mug79+ (spo7+)SPAC6G9.04Meiotic spindle pole body component
 spo15SPAC1F3.06cMeiotic spindle pole body component
Class 4. Genes that are essential for the proper formation and the maturation of the forespore membrane (n = 15)
 csn1+SPBC215.03cCOP9/signalosome complex subunit
 csn2+SPAPB17E12.04cCOP9/signalosome complex subunit
 cdt2+SPAC17H9.19cCOP9/signalosome associated factor
 erp2+SPAC17A5.08ER exit receptor for secretory cargo
 erp5+SPBC16E9.09cER exit receptor for secretory cargo
 mes1+SPAC5D6.08cMeiotic APC/C regulator
 spe2+SPBP4H10.05cS-adenosylmethionine decarboxylase proenzyme
 (spe3+)SPBC12C2.07cPredicted spermidine synthase
 spo3+SPAC607.10Required for spore formation
 spo4+SPBC21C3.18Kinase required for spore formation
 spo5+SPBC29A10.02Meiotic RNA-binding protein
 tpp1+SPAC19G12.15cTrehalose-6-phosphate phosphatase
SPAC6C3.06cPredicted P-type phospholipid flippase
SPCC1739.04cSequence orphan
  • ER, endoplasmic reticulum; APC/C, Anaphase Promoting Complex/Cyclosome.

  • a Descriptions are based on PomBase (Wood et al. 2012) (

  • b Knockout not confirmed by barcode sequencing.