Table 4 Known sporulation-defective genes listed in the S. pombe haploid deletion set
GeneCommentPhenotype in Our Study
spo3+Confirmed by barcode sequenceSporulation defect
spo4+Confirmed by barcode sequenceSporulation defect
spo5+Knockout found at different position in the collectionaSporulation defect
spo6+Not presentan.d.b
mug79+/spo7+Confirmed by barcode sequenceSporulation defect
spo15+Confirmed by barcode sequenceSporulation defect
mei2+Confirmed by barcode sequenceSporulation defect
mei3+Confirmed by barcode sequenceSporulation defect
mei4+Knockout found at different position in the collectionaSporulation defect
mes1+Confirmed by barcode sequenceSporulation defect
mfr1+Not presentan.d.
meu14+Knockout is present as determined both by PCR with flanking primers and by barcode sequenceNormal sporulation
cdt2+Confirmed by barcode sequenceSporulation defect
  • n.d., not determined; PCR, polymerase chain reaction.

  • a See Table 3.