Table 1 Frequencies of vasa-Cas9/gRNA–mediated mutagenesis
LocusgRNAF0Fertile F0 with Somatic Mutation n/n, (%)F1 Mutation Rates n/n, (%)
ms(3)k81InjectionFemale20/26 (76.9)11/49 (22.4)
Male12/17 (70.1)5/20 (25.0)
U6B-k81Female22/22 (100)4/28 (14.3)
Male26/26 (100)aNA
YellowU6B-y1Female36/36 (100)221/588 (37.6)
Male8/8 (100)158/178 (88.8)b
CR7T-y2Female30/30 (100)593/628 (94.4)
Male7/7 (100)74/82 (90.2)b
WhiteCR7T-w1Female23/23 (100)NA
Male17/17 (100)430/664 (64.8)
CR7T-w2Female23/23 (100)NA
Male18/18 (100)86/506 (17.0)
  • NA, not applicable.

  • a All males are sterile.

  • b The female progeny of F1 was counted as total progeny.