Table 1

Mutants identified from the screen

geneallelesgrk mRNA localization in stage 6grk mRNA localization in stage 9other phenotypes
mislocalised grk mRNA in late stagesarmitage (armi)3R13, 9R1, 10M12, 11R3, 11S9, 13P2, 13P5, 14P4diffuses internallyweakly accumulates around the nucleus, spreads along anterior peripherydefective follicle cell development
saturn*: kinesin light chain (klc) & maelstrom (mael)5R12diffuses internallyno longer localizes to the anterior periphery nor to the nucleusmispositioned nucleus
scaf6 + unmapped mutation12M9diffuses internallyweakly accumulates around the nucleus, spreads along anterior peripherydefective reproduction of germline stem cells
elg13R7localizes posteriorly as in wild typespreads along anterior peripherydefective nurse cell DNA endoreplication
Phenotypes
other mutantsnudE11R2Oocyte is misspecified. Most egg chambers contain 16 nurse cells.
quemao (qm)5R4, 11S5Plasma membranes of nurse cells and the oocyte collapse. Released ring canals cluster. grk mRNA colocalizes to the ring canal cluster.
Tsc2/gigas1R8, 2R5, 11R10Oocyte is frequently mispositioned.
rasp11R5, 13P2, 13P10, 13P13Mature eggs have fused appendages. Gurken proteins diffuse in the cytoplasm.
Chd5/Wrb9R9, 13P2Disorganized nurse cells in late stage 10.
  • * saturn (5R12) is a synthetic phenotype caused by mutations in klc and mael (see Results).