Table 4 Cuticle permeability assay
StrainHoechst % Stained
N25%
bar-1(ga80)25%**
bli-1(e769)54%**
tag-164(ok771)8%
sptf-2(tm1130)6%
vector control (RNAi)4%
col-38(RNAi)17%**
col-49(RNAi)25%**
col-71(RNAi)7%
dao-4(RNAi)15%**
  • Cuticle permeability was tested by a 15-min exposure to Hoechst dye in control animals (N2, vector control), animals carrying a bar-1 loss-of-function mutation, or animals with reduced function for Wnt target genes (mutants for bli-1, tag-164(ok771), and sptf-2; RNAi treatment for col-38, col-49, col-17, and dao-4). The percent of animals with significant nuclear staining after a 15-min treatment is indicated. RNAi, RNA interference.

  • ** P < 0.0001, Fisher t-test