Table 4 Restriction sites found in the multiple cloning sites of the pCU and pCN plasmids
PromoterMCSOther Sites
pCU seriesEGD2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
HHT2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
PDC1XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
ACO2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
LYS21SpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
MET3XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
pCN seriesEGD2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
HHT2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
PDC1XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
ACO2XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
LYS21SpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
MET3XbaISpeIBamHISmaIPstIEcoRIEcoRVHindIIIClaISalIXhoISacIKpnI
  • Bold lettering indicates the sequence is unique in that particular plasmid. Sites listed under the MCS heading are found in the multiple cloning site of the plasmid; SacI and KpnI are located at the beginning of the promoter and end of the HIS3 terminator, respectively. MCS, multiple cloning site.