Table 2 Plasmids used in this study
Plasmid NameDescriptionParent VectorBacterial StockSourceGenbank AccessionAddgene ID
General use
pGRB2.0CEN/ARS plasmid [ApR, URA3]pRS406b65G. Rotano, unpublishedKF04039445340
pGRB2.1CEN/ARS plasmid containing HIS3 3′ UTR [ApR, URA3]pGRB2.0b54Frieman et al. 2002KF04039545341
pGRB2.2PGK1pr on CEN/ARS plasmid also containing HIS3 3′ UTR [ApR, URA3]pGRB2.1b162Frieman et al. 2002KF04039645342
pGRB2.3GFP driven by PGK1pr on CEN/ARS plasmid [ApR, URA3]pGRB2.2b164This workKF04039745343
pBM16CEN/ARS plasmid containing NATR cassette [ApR, NATR]pUC19b1564Ma et al. 2009KF04039845344
pBM16.1CEN/ARS plasmid containing NATR cassette; same backbone as pBM16, with more limited MCS. [ApR, NATR]pBM16b1879This workKF04039945345
Constitutive promoters
pCU-EGD2EGD2pr empty vector [ApR, URA3]pGRB2.1b2448This workKF04037045315
pCU-EGD2-GFPEGD2pr-GFP [ApR, URA3]pGRB2.3b2037This workKF04037145316
pCN-EGD2EGD2pr empty vector [ApR, NATR]pBM16.1b2454This workKF04037245317
pCN-EGD2-GFPEGD2pr-GFP [ApR, NATR ]pBM16b2236This workKF04037345318
pCU-HHT2HHT2pr empty vector [ApR, URA3]pGRB2.1b2449This workKF04037445319
pCU-HHT2-GFPHHT2pr-GFP [ApR, URA3]pGRB2.3b2038This workKF04037545320
pCN-HHT2HHT2pr empty vector [ApR, NATR]pBM16.1b2455This workKF04037645321
pCN-HHT2-GFPHHT2pr-GFP [ApR, NATR]pBM16b2238This workKF04037745322
pCU-PDC1PDC1pr empty vector [ApR, URA3]pGRB2.1b2450This workKF04037845323
pCU-PDC1-GFPPDC1pr-GFP [ApR, URA3]pGRB2.3b2039This workKF04037945324
pCN-PDC1PDC1pr empty vector [ApR, NATR]pBM16.1b2456This workKF04038045325
pCN-PDC1-GFPPDC1pr-GFP [ApR, NATR]pBM16b2240This workKF04038145326
Macrophage-induced promoters
pCU-ACO2ACO2pr empty vector [ApR, URA3]pGRB2.1b2451This workKF04038245327
pCU-ACO2-GFPACO2pr-GFP [ApR, URA3]pGRB2.3b2230This workKF04038345328
pCN-ACO2ACO2pr empty vector [ApR, NATR]pBM16.1b2457This workKF04038445329
pCN-ACO2-GFPACO2pr-GFP [ApR, NATR]pBM16b2235This workKF04038545330
pCU-LYS21LYS21pr empty vector [ApR, URA3]pGRB2.1b2452This workKF04038645331
pCU-LYS21-GFPLYS21pr-GFP [ApR, URA3]pGRB2.3b2232This workKF04038745332
pCN-LYS21LYS21pr empty vector [ApR, NATR]pBM16.1b2458This workKF04038845334
pCN-LYS21-GFPLYS21pr-GFP [ApR, NATR]pBM16b2239This workKF04038945335
Nutritionally regulated promoters
pCU-MET3MET3pr empty vector [ApR, URA3]pGRB2.1b2453This workKF04039045336
pCU-MET3-GFPMET3pr-GFP [ApR, URA3]pGRB2.3b1971This workKF04039145337
pCN-MET3MET3pr empty vector [ApR, NATR]pBM16.1b2459This workKF04039245338
pCN-MET3-GFPMET3pr-GFP [ApR, NATR]pBM16b2410This workKF04039345339
  • UTR, untranslated region; NATR, nourseothricin-resistant.