Table 2 Phenotypic analysis of specific histone H3 and histone H4 mutants
Phenotypes of Strains Obtained from the Histone Mutant LibraryaPhenotypes of Strains in Which Histone Mutants Are Expressed from Plasmidsa
YPDYPDYPDYPDYPDYPDYPDYPDYPDYPD
Histone14°Caff.Form.HU14°Caff.Form.HUPhenotypes, Dai et al.b
WT5c555555555
H3-K18Q4434555555
H3-R49A5204332012HUs
H3-G44A5414221001HUs
H4-K44A5334354355
H4-R36A5012130202Cs, HUs
H4-K31E4414154555n/d
  • a Phenotypes were scored based on data obtained from spot tests similar to those shown in Figure 3. Refer to the text for a description of the difference between the two sets of strains indicated here.

  • b See Dai et al. (2008) and Huang et al. (2009). The only phenotypes considered here are cold-sensitivity (scored at 16°) and HU-sensitivity conferred by the indicated mutants when integrated into the genome and/or expressed from a plasmid. Dai et al. (2008) did not score for caffeine or formamide sensitivity.

  • c Growth was scored on a scale of 5 to 0, with 5 indicating robust growth similar to that seen in wild-type cells and 0 indicating no detectable growth.