Table 1 Effect of donor homology length
Donor LengthHostParentsYieldersMutantsNo. of Mutants/ParentTestedHR% HR
0.2 kbCS84182462.911000
lig41099860.83400
0.5 kbCS108274153.816632
lig4167342781.71553724
1.0 kbCS73224095.6122108
lig4111232582.31052524
2.1 kbCS120477366.12405021
lig4155261891.21086762
4.2 kbCS489275674513.8123125220
lig446215236887.999856757
7.5 kbCS296151411113.98528410
lig42216815427.036922661
  • Donors of the indicated lengths were tested in wild-type Canton S (CS) flies or a lig4 mutant. The total number of parents tested, number of those yielding mutants, and total number of mutants recovered are listed, as is the average number of mutants per parent. The number of individual mutants tested for NHEJ vs. HR is shown, then the number of those that incorporated the donor sequence (HR). Finally the percentage of those tested that were products of HR is listed (HR/tested × 100).