Table 3 puf-8 is unlikely to function in the gld-1 or gld-2 pathways
GenotypeLength Proliferative Zone (Cell Diameter, Range)a
Wild type (N2)20 (15-24)
puf-8(oz192)13 (7−18)
gld-1(q485)14 (10-18)b
gld-2(q497)27 (23-33)c
gld-3(q730)27 (23-32)d
nos-3(oz231)20 (14-24)c
gld-1(q485); puf-8(oz192)14 (11-16)e
gld-2(q497); puf-8(oz192)19 (15-22)
gld-3(q730) puf-8(oz192)18 (16-20)
puf-8(oz192) nos-3(oz231)14 (11-17)
  • a Animals were dissected 1 d past the L4 stage and gonads were fixed and stained with DAPI, anti-REC-8 antibodies and anti-HIM-3 antibodies to visualize nuclear morphology and to determine the extent of proliferation vs. meiotic entry. The length of the proliferative zone for each double mutant was counted for a minimum of five gonad arms to obtain an average. The range of results obtained is shown. At least 20 additional gonad arms were analyzed in each double mutant, none of which showed evidence of over-proliferation.

  • b In gld-1(q485) animals, germ cells enter into meiosis normally; however, female germ cells are unable to complete meiosis. Rather, these cells re-enter the mitotic cell cycle, forming a tumor in the proximal end of the gonad. Data obtained from Eckmann et al. 2004.

  • c Data obtained from Hansen et al. 2004b.

  • d Data obtained from Eckmann et al. 2004.

  • e Whereas gld-1(q485) single mutants are feminized, with female germ cells failing to progress through meiotic prophase, instead returning to the mitotic cell cycle and forming a proximal tumor, gld-1(q485); puf-8(oz192) animals make sperm. Therefore, puf-8(oz192) suppresses gld-1(q485) germline feminization. A total of 76% (n = 35) of gonad arms also contained proximal proliferation typical of gld-1(q485) single mutants. gld-1(q485); puf-8(q725); glp-1(q175) are also Glp (n = 72), further supporting that the proximal proliferation in the 76% of gonad arms is due to de-differentiation rather than a synthetic interaction between gld-1 and puf-8.