Table 2 FISH-validated genes required for normal somatic homolog pairing
Gene TargetedGene FunctionValidation Amplicon% Paired NucleiaNuclei ScoredNuclear Sizeb% Nuclei > 4 Signalsc
Control(water)66.844982537 ± 8920.002
β-Tub56DCytoskeletonDRSC3323647.93612943 ± 13301.1
CG31635UnknownDRSC31568542742573 ± 7870.36
CG5844Fatty acid metabolismDRSC3097752.31512417 ± 9250
chbMicrotubule bindingDRSC3646457.73452643 ± 8320.29
feoCytokinesisDRSC2377353.12412892 ± 15983.7
gemininRegulation of DNA replicationDRSC3331156.31903879 ± 18862.1
His3Core histoneDRSC4090457.61842848 ± 10410.54
ial-1Aurora B kinase; cytokinesisDRSC3631145.42073360 ± 20486.3
IncenpInner centromere proteinDRSC3934346.61613637 ± 22979.9
Klp61FMitotic kinesinDRSC3082331.71233727 ± 196713.9
MybOncogeneDRSC3109939.72093315 ± 184312
pavKinesin; cytokinesisDRSC3333333.81362972 ± 19038.8
pblCytokinesisDRSC3333541.52992776 ± 12293.7
poloKinetechoreDRSC3446346.72442628 ± 11802
scraContractile ringDRSC0767941.42803313 ± 18078.2
SseMitotic chromosome segregationDRSC3447653.63082777 ± 12290.32
tsrActin binding proteinDRSC3127755.61442289 ± 12564.1
  • a Percentage of nuclei with a single FISH signal for the 359 bp repeat.

  • b Nuclear sizes were determined from images representing flattened z-stacks and are presented as mean areas of DAPI staining plus or minus the standard deviation. Units are arbitrary.

  • c Percentage of nuclei with greater than four FISH signals for the 359 bp repeat, which are assumed to be polyploid/aneuploid.