Table 2  Summary of RNA inference effects
Phenotypic Penetrance
dsRNA SequencedsRNA Size, bpTarget Gene KnockdownaNumber ScoredLegsDefects in Other Appendages
UnaffectedMildModerateSevere
GFP6008396.4%2.4%b01.2%b1.2%b
dpp81266% ± 13%*3537%17%17%29%40%
Dll46275% ± 4.9%*4217%4.8%17%62%91%
dac35930% ± 16%6638%11%23%29%76%
hth33544% ± 7.5%*20534%39%22%6%90%
Lim138856% ± 31%1631%6.3%25%38%75%
Notch40924% ± 9.0%*3123%6.5%19%52%94%
Ser329n/ac15114%0.7%2.6%83%97%
Delta18071% ± 14%*7585%6.7%6.7%1.3%38%
Ser, Delta2344%04.3%52%78%
odd21165% ± 7.3%*5036%14%8.0%42%86%
bowl34263% ± 5.2%*5746%8.8%8.8%37%79%
sob32161% ± 8.0%*166.3%056%38%94%
drm225n/ac303.3%03.3%93%100%
odd, bowl, sob1650%6.3%19%25%81%
odd, bowl, sob, drm375.4%5.4%089%100%
Krn18867% ± 9.2%*1911%5.3%21%63%90%
bab774n/ac2218%4.5%9.1%68%68%
ab19842% ± 6.3%*3126%6.5%068%81%
rn12778% ± 9.0%*5275%2%14%10%21%
ss355n/ac1958%16%5.3%21%100%
total107633%76%
  • The level of target gene knockdown was determined by real-time PCR comparisons of pooled pupae to nonspecific GFP dsRNA controls. The phenotypic effects on legs and other appendages were scored after metamorphosis.

  • * Significant difference from gene expression in GFP control specimens (Welch’s t-test, P < 0.05).

  • a 0% represents no reduction in activity, while 100% is complete suppression.

  • b One control specimen eclosed with truncated T1 legs (scored as severe). Two specimens were missing a single tarsal joint. One specimen had a fusion of the distal- most segments of one antenna.

  • c Suitable primers for this gene were unavailable (i.e., n/a).