Table 2 Summary of sequence reads generated from mutant and wild-type samples

Sample	Total No. of Reads	Read Length in Bases	Total Mb	Coverage	No. of Reads Mapped to Reference Genome	% of Reads Mapped to Reference Genome
Wild type	28,760,231	76	2186	54x	17,322,318	60.2
pro23/fus	23,164,874	76	1761	44x	16,616,121	71.7
fus	56,139,346	76	4267	107x	48,548,370	86.5
pro44	86,849,208	50	4342	109x	73,788,312	85.0

The total number of reads is the number after removing reads containing undefined bases (“N”). Reads were mapped to the S. macrospora reference genome (Nowrousian et al. 2010) using the Burrows-Wheeler Alignment tool (BWA) (Li and Durbin 2009).