Sample | Total No. of Reads | Read Length in Bases | Total Mb | Coverage | No. of Reads Mapped to Reference Genome | % of Reads Mapped to Reference Genome |
---|---|---|---|---|---|---|
Wild type | 28,760,231 | 76 | 2186 | 54x | 17,322,318 | 60.2 |
pro23/fus | 23,164,874 | 76 | 1761 | 44x | 16,616,121 | 71.7 |
fus | 56,139,346 | 76 | 4267 | 107x | 48,548,370 | 86.5 |
pro44 | 86,849,208 | 50 | 4342 | 109x | 73,788,312 | 85.0 |
The total number of reads is the number after removing reads containing undefined bases (āNā). Reads were mapped to the S. macrospora reference genome (Nowrousian et al. 2010) using the Burrows-Wheeler Alignment tool (BWA) (Li and Durbin 2009).