Table 1  Primers used for detecting allelic variation in VRN-A1 and VRN-B1 in tetraploid wheat
PrimerSequence (5′–3′)PositionReference
LociGene Region
VRN1AFGAAAGGAAAAATTCTGCTCGVRN-A1PromoterYan et al. (2004a)
VRN1ARTGCACCTTCCC(C/G)CGCCCCATVRN-A1Promoter
Ex1/C/FGTTCTCCACCGAGTCATGGTVRN-A1Intron 1Fu et al. (2005)
Intr1/A/R3AAGTAAGACAACACGAATGTGAGAVRN-A1Intron 1
Intr1/C/FGCACTCCTAACCCACTAACCVRN-A1Intron 1
Intr1/AB/RTCATCCATCATCAAGGCAAAVRN-A1Intron 1
Intr1/B/FCAAGTGGAACGGTTAGGACAVRN-B1Intron 1
Intr1/B/R3CTCATGCCAAAAATTGAAGATGAVRN-B1Intron 1
Intr1/B/FCAAGTGGAACGGTTAGGACAVRN-B1Intron 1
Intr1/B/R4CAAATGAAAAGGAATGAGAGCAVRN-B1Intron 1
VRNBPF1CCCCTGCTACCAGTGCCTACTAVRN-B1960 bp upstream from start codonNewly developed
VRNBPF2GGCTTGGGGTGTAGGGTTGGVRN-B118 bp upstream from start codon
VRNBPF3CCCTCTCTTCCGCCTCACCCAACVRN-B1116 bp upstream from start codon
VRNBPR1GCCCCATCTCCGCTGGAGAACGVRN-B1Contained start codon
VRNBPR2CAGGTGGTTGGGTGAGGCGGAAGVRN-B1110 bp upstream from start codon
VRNBPR3CGTAAATATCCCCAGCCAGAVRN-B1586 bp downstream from start codon
VBINSR1CCCGCTTGTTGGCTGGTGAGVRN-B142 bp downstream from VRNBPF3 in PI 94749