Table 2  Mating and cross efficiency between knockout strains from a library plate and a rΔ tester (F2-27 or F2-29)
57 mat a strains crossed to the rΔA tester
No. of strains at a given perithecial levelNo. of strains at a given ascospore level
P>50 = 44A>200 = 53
P≤50 = 11A≤200 = 1
P≤20 = 2A≤50 = 3
P0 = 0A0 = 0
39 mat A strains crossed to the rΔa tester
No. of strains at a given perithecial levelNo. of strains at a given ascospore level
P>50 = 37A>200 = 38
P≤50 = 1A≤200 = 0
P≤20 = 1A≤50 = 0
P0 = 0A0 = 1
  • Perithecial and ascospore productivity were scored on a 4-level scale. For example, 44 mat a knockout strains yielded >50 perithecia when mated with the rΔ A tester.