Table 2  Proportions of cells with foci in exponential, stationary, and late-stationary phases
Exponential PhaseStationary PhaseLate-Stationary Phase
Harvested 6.5–8.5 hrHarvested 8.5–11 hrHarvested 21 hr
% of cells with fociN% of cells with fociN% of cells with fociN
Wild-type9916971731740808
recB21915381729630454
recG258981019186330262
ruvA6091620213700379
recG258 ruvA60741392114910327
  • Cultures were grown, exposed to EdU, harvested, and visualized as described in Materials and Methods. “Harvested” refers to the time (in hours) after inoculation that cells were collected and fixed. These times are greater than the harvesting times for the flow cytometry samples because the cultures were started with smaller inocula. For each sample taken from the exponential phase and stationary phase, three fields were imaged, and the total number of cells with fluorescent foci was counted. For each sample taken in late-stationary phase, three fields were imaged, but the total number of cells in only one field was counted. Wild-type = FC36; recB21= FC400; recG258 = FC457; ruvA60 = FC484; recG258 ruvA60 = FC513.