Table 3  Silencing by ectopic transgenes and its suppression
Parent 1 (mat A): mutation at sad-1 or sad-3Parent 2 (mat a): ectopic insertion at his-3 (::)Unpaired geneAscospore count (× 103)
Wild typeNo insertions415
::act+actin28.8
::BmlRβ-tubulin0.612
sad-1No insertions-209
::act+actin358
::BmlRβ-tubulin399
sad-3No insertions-29.9
::act+actin74.3
::BmlRβ-tubulin59.7
  • Meiotic silencing of unpaired actin or β-tubulin leads to the reduced production of ascospores. Such reduction is not observed in crosses carrying sad-1 or sad-3. Strains used in this experiment include F2-14, F2-37, F2-38, P3-07, P8-18, and P14-28.