Table 1  Genetic characteristics of 73 Hsp introgression lines (S42ILs) based on genotyping with 636 Illumina SNPs
S42ILaChromosomebPosition of Start SNPcPosition of End SNPdSize of Target IntrogressioneNo. of Additional IntrogressionsfHsp (%)gHeterozygous (%)hS42IL-HR (g)i
−1011H1.1013.5017.731.12.2715
−102*1.1098.2399.225.22.2673
−10340.5189.0151.133.03.1968
−15764.7990.9228.612.30.0i.p.
−10470.7878.0311.921.90.0703
−10574.4090.9218.501.20.0293
−15882.3590.9210.511.90.0i.p.
−141*94.86127.7136.323.00.2504
−143*130.68173.4951.844.21.61180
−142*188.50205.0724.101.50.0348
−106*2H22.3534.3119.101.20.0865
−10734.3166.7842.013.30.0607
−108*34.31104.8177.915.00.8290
−109*63.96110.8442.403.30.0984
−153*108.71120.8313.331.80.31183
−14463.9681.5021.311.40.0421
−110*102.66104.815.721.40.047
−175*197.39247.8652.643.51.8i.p.
−111*3H67.0198.4134.943.71.91062
−154*64.85144.3084.826.92.3i.p.
−155104.39144.3046.745.50.5i.p.
−112*104.39161.4364.313.81.71312
−159154.99190.8740.114.90.0i.p.
−114138.00245.49109.734.63.91392
−140*154.99253.73101.006.20.02096
−115*204.48255.1353.714.30.0791
−160204.48221.4324.812.50.0i.p.
−113204.48239.7338.302.40.0396
−161239.73253.7319.101.20.0i.p.
−116*4H5.4247.8049.125.30.0724
−117*27.5264.7741.202.60.0529
−14561.1564.7713.821.61.01073
−118*61.1583.5832.212.50.0957
−12061.1583.5832.201.40.71352
−11961.15119.0669.514.90.0978
−16261.15119.0669.504.40.0No
−16461.1599.7449.313.50.029
−12174.11119.0650.623.62.1952
−146*83.58119.0643.101.90.61322
−16691.93119.0632.012.50.0No
−123*128.85172.3250.535.50.01174
−124*171.25183.5413.422.40.01339
−173*5H104.73171.3474.104.70.0i.p.
−125104.73154.3756.013.60.51095
−147145.57154.3717.311.10.2i.p.
−126145.57200.1261.803.90.0400
−176*154.37234.9881.836.30.8No
−127*231.75276.7750.003.20.01349
−1486H3.2810.7312.723.50.11519
−15073.9082.4313.511.30.01167
−15271.3982.4331.233.50.3i.p.
−14971.3982.4331.212.40.01659
−12871.39132.2377.815.20.31394
−12973.90133.4763.003.50.5303
−156*89.78156.0972.138.41.555
−13098.66180.6983.824.91.11250
−131*140.00180.6947.202.30.71244
−163137.78163.5631.122.00.2i.p.
−132160.38191.4631.602.00.0185
−122*180.69208.1332.833.51.21007
−15198.66111.5616.532.20.71419
−133*7H17.3251.9344.044.90.7302
−134*51.93107.4459.113.70.91468
−135*101.23152.2963.104.00.029
−167101.23114.5814.410.90.1i.p.
−168101.23116.6816.521.51.640
−169101.23120.9226.421.70.817
−170114.58120.9213.100.80.0i.p.
−171116.68134.4319.511.30.242
−136134.43152.2936.723.50.01137
−137*134.43193.8966.848.10.2948
−138*176.37229.6665.314.81.1841
−139198.70229.6631.502.00.0474
Average42.91.53.30.6
  • a Order of the lines follows chromosomal position of the target introgression and corresponds to the order in Table S1. The 32 lines of the S42IL core set are indicated by asterisks (*).

  • b Chromosomal location of the target introgression, based on Close et al. (2009).

  • c Chromosomal position of the first marker of the target introgression (in cM).

  • d Chromosomal position of the last marker of the target introgression (in cM).

  • e Estimated size of the target introgression (in cM).

  • f Number of Hsp segments, additional to the target introgression.

  • g Percentage of homozygous Hsp genome per line, based on a total genome size of 1576 cM.

  • h Percentage of heterozygous loci per line.

  • i Grams (g) of seed available for each BC4S2 population. An HR population consisting of 400 g of seed corresponds to 10,000 segregating BC4S2 individuals assuming an average thousand grain weight of 40g (Schmalenbach et al. 2009). The abbreviation “i.p.” indicates that the HR population is currently in preparation through field multiplication.