Table 2 Genome assembly summary statistics, before and after manual ordering of scaffolds

	Unordered Assembly (Scaffolds)				Ordered Assembly (Ultra-scaffolds)				Percentage of Assembly^a
	Number	Bases	N50	Gaps	Number	Bases	N50	Gaps	Percentage of Assembly^a
S. bayanus (CBS 7001)	629 (147)	11,668,028	444,551	380	16	11,467,582	905,555	394	98.3%
S. kudriavzevii (IFO 1802^T)	1455 (226)	11,736,856	151,185	17	16	11,294,830	882,337	111	96.2%
S. kudriavzevii (ZP 591)	1523 (164)	11,642,553	100,201	10	16	11,185,947	882,203	162	96.2%
S. mikatae (IFO 1815^T)	1220 (159)	11,922,798	360,232	18	16	11,445,471	800,823	52	96.0%

Numbers in parentheses indicate scaffolds longer than 500 bp.
↵a Percentage of base pairs in the unordered assembly that are also present in the ordered assembly. Neither contigs with an average Kmer coverage less than 20 nor gaps in scaffolds (i.e., N bases) were counted toward assembly statistics.