Table 6 Construction of heterothallic haploid strains with auxotrophic markers for S. mikatae, S. kudriavzevii and S. bayanus

Species	Strain	Original	Genotype	Reference
S. mikatae	JRY9171	IFO 1815^T	MATa hoΔ::KanMX ura3Δ::HygMX	This study
S. mikatae	JRY9172	IFO 1815^T	MATα hoΔ::KanMX ura3Δ::HygMX	This study
S. mikatae	JRY9173	IFO 1815^T	MATa hoΔ::NatMX ura3Δ::HygMX	This study
S. mikatae	JRY9174	IFO 1815^T	MATα hoΔ::NatMX ura3Δ::HygMX	This study
S. mikatae	JRY9175	IFO 1815^T	MATa hoΔ::KanMX his3Δ::HygMX	This study
S. mikatae	JRY9176	IFO 1815^T	MATα hoΔ::NatMX trp1Δ::HygMX	This study
S. mikatae	JRY9177	IFO 1815^T	MATa hoΔ::KanMX his3Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9178	IFO 1815^T	MATα hoΔ::KanMX his3Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9179	IFO 1815^T	MATa hoΔ::NatMX his3Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9180	IFO 1815^T	MATα hoΔ::NatMX his3Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9181	IFO 1815^T	MATa hoΔ::KanMX trp1Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9182	IFO 1815^T	MATα hoΔ::KanMX trp1Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9183	IFO 1815^T	MATa hoΔ::NatMX trp1Δ::HygMX ura3Δ::HygMX	This study
S. mikatae	JRY9184	IFO 1815^T	MATα hoΔ::NatMX trp1Δ::HygMX ura3Δ::HygMX	This study
S. kudriavzevii	FM1097	IFO 1802^T	MATα hoΔ::natMX	Hittinger et al. 2010
S. kudriavzevii	FM1098	IFO 1802^T	MATa hoΔ::natMX	Hittinger et al. 2010
S. kudriavzevii	FM1363	IFO 1802^T	MATα hoΔ::kanMX	This study
S. kudriavzevii	FM1403	IFO 1802^T	MATa/MATα hoΔ::kanMX/hoΔ::kanMX	This study
S. kudriavzevii	FM1122	IFO 1802^T	MATα hoΔ::natMX ura3Δ0	This study
S. kudriavzevii	FM1141	IFO 1802^T	MATα hoΔ::natMX ura3Δ0 trp1Δ::ScerURA3⁺	This study
S. kudriavzevii	FM1388	IFO 1802^T	MATα hoΔ::natMX ura3Δ0 his3Δ0	This study
S. kudriavzevii	JRY9185	IFO 1802^T	MATa hoΔ::natMX ura3Δ0	This study
S. kudriavzevii	JRY9186	IFO 1802^T	MATα hoΔ::natMX trp1Δ0	This study
S. kudriavzevii	JRY9187	IFO 1802^T	MATa hoΔ::natMX trp1Δ0 ura3Δ0	This study
S. kudriavzevii	JRY9188	IFO 1802^T	MATα hoΔ::natMX trp1Δ0 ura3Δ0	This study
S. kudriavzevii	FM1109	ZP 591	MATa hoΔ::kanMX	Hittinger et al. 2010
S. kudriavzevii	FM1110	ZP 591	MATα hoΔ::kanMX	Hittinger et al. 2010
S. kudriavzevii	FM1071	ZP 591	MATa/MATα	Hittinger et al. 2010
S. kudriavzevii	FM1158	ZP 591	MATa/MATα	This study
S. kudriavzevii	FM1400	ZP 591	MATa/MATα hoΔ::kanMX/hoΔ::kanMX	This study
S. kudriavzevii	FM1340	ZP 591	MATa hoΔ::natMX ura3Δ0	Hittinger et al. 2010
S. kudriavzevii	FM1123	ZP 591	MATa hoΔ::kanMX ura3Δ0	Hittinger et al. 2010
S. kudriavzevii	FM1192	ZP 591	MATα hoΔ::kanMX ura3Δ0	This study
S. kudriavzevii	FM1194	ZP 591	MATa hoΔ::kanMX trp1Δ0	This study
S. kudriavzevii	FM1131	ZP 591	MATα hoΔ::kanMX trp1Δ0	Hittinger et al. 2010
S. kudriavzevii	FM1183	ZP 591	MATa hoΔ::kanMX ura3Δ0 trp1Δ0	Hittinger et al. 2010
S. kudriavzevii	FM1193	ZP 591	MATα hoΔ::kanMX ura3Δ0 trp1Δ0	This study
S. kudriavzevii	FM1389	ZP 591	MATa hoΔ::kanMX ura3Δ0 his3Δ0	This study
S. bayanus	JRY9189	CBS 7001	MATa hoΔ::NatMX	This study
S. bayanus	JRY9190	CBS 7001	MATα hoΔ::NatMX	This study
S. bayanus	JRY8149	CBS 7001	MATa hoΔ::NatMX his3 lys2 ura3	Gallagher et al. 2009
S. bayanus	JRY8150	CBS 7001	MATα hoΔ::NatMX his3 lys2 ura3	Gallagher et al. 2009
S. bayanus	JRY8153	CBS 7001	MATa hoΔ::NatMX his3 lys2 trp ura3	Gallagher et al. 2009
S. bayanus	JRY8154	CBS 7001	MATα hoΔ::NatMX his3 lys2 trp ura3	Gallagher et al. 2009
S. bayanus	JRY8147	CBS 7001	MATa hoΔ::NatMX ade2 his3 lys2 ura3	Gallagher et al. 2009
S. bayanus	JRY8148	CBS 7001	MATα hoΔ::NatMX ade2 his3 lys2 ura3	Gallagher et al. 2009
S. bayanus	JRY9191	CBS 7001	MATa hoΔ::NatMX his3 ura3	This study
S. bayanus	JRY9040	CBS 7001	MATa hoΔ::NatMX lys2 ura3	Zill et al. 2010
S. bayanus	JRY9192	CBS 7001	MATa hoΔ::NatMX ade2 ura3	This study
S. bayanus	JRY9193	CBS 7001	MATα hoΔ::NatMX ade2 ura3	This study
S. bayanus	JRY9194	CBS 7001	MATa hoΔ::loxP his3 lys2 ura3	This study
S. bayanus	JRY9195	CBS 7001	MATα hoΔ::loxP his3 lys2 ura3	This study

All strains are available upon request from C. T. Hittinger.