RT Journal Article SR Electronic T1 Genetic and Physical Localization of the Gene Controlling Leaf Pigmentation Pattern in Medicago truncatula JF G3: Genes|Genomes|Genetics FD Genetics Society of America SP 4159 OP 4165 DO 10.1534/g3.120.401689 VO 10 IS 11 A1 Yu, Xiaocheng A1 Qin, Qiulin A1 Wu, Xia A1 Li, Dandan A1 Yang, Shengming YR 2020 UL http://www.g3journal.org/content/10/11/4159.abstract AB In Medicago truncatula, some ecotypes form a black or purple stain in the middle of adaxial leaf surface due to accumulation of anthocyanins. However, this morphological marker is missing in some other ecotypes, although anthocyanin biosynthesis pathway is not disrupted. Genetic analysis indicated that the lack of the leaf spot of anthocyanins accumulation is a dominant trait, which is controlled by a single gene, LPP1. Genetic mapping indicated that the LPP1 gene was delimited to a 280 kb-region on Chromosome 7. A total of 8 protein-coding genes were identified in the LPP1 locus through gene annotation and sequence analysis. Of those, two genes, putatively encoding MYB-transcriptional suppressors, were selected as candidates for functional validation.