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Research ArticleInvestigation

Rapid Nuclear Exclusion of Hcm1 in Aging Saccharomyces cerevisiae Leads to Vacuolar Alkalization and Replicative Senescence

Ata Ghavidel, Kunal Baxi, Martin Prusinkiewicz, Cynthia Swan, Zach R. Belak, Christopher H. Eskiw, Carlos E. Carvalho and Troy A. Harkness
G3: Genes, Genomes, Genetics March 2018, g3.200161.2018; DOI: https://doi.org/10.1534/g3.118.200161
Ata Ghavidel
University of Saskatchewan
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Kunal Baxi
University of Saskatchewan
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Martin Prusinkiewicz
University of Saskatchewan
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Cynthia Swan
University of Saskatchewan
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Zach R. Belak
University of Saskatchewan
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Christopher H. Eskiw
University of Saskatchewan
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Carlos E. Carvalho
University of Saskatchewan
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Troy A. Harkness
University of Saskatchewan
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  • For correspondence: troy.harkness@usask.ca

Supplemental Material for Ghavidel et al., 2018

  • Figure S1 -

    (A)Live cell imaging of dividing WT yeast.  (B) Live cell imaging of dividing yeast co-stained with the vacuolar membrane marker FM4-64 and calcofluor-white. (C) cDCFDA fluorescence emission spectra of young and old yeast. (D)Live cell imaging of dividing Matα yeast cells expressing either Ste3-GFP, or the GFP variant pHluorin fused to the
    pheromone receptor Ste3. (.pptx)

  • Figure S2 -

    (A)Expression ratios of RT-PCR RNA expression analyses of vacuolar proton pump subunits (VMA) and a vacuolar fusion factor (YPT7) in replicatively old cells enriched using MEP.  (B) CMAC-Arg fluorescence emission spectra of cells in (Fig. 2B) (n=25).(.pptx)

  • Figure S3 -

    (A) Live cell microscopy of WT yeast expressing a genomically integrated gal-inducible α-syn-GFP construct. (B) RNA expression analysis of autophagosome (ATG) subunits in early log (young) cells and a population enriched for replicatively old cells using MEP. (.pptx)

  • Figure S4 -

    Consensus binding motifs for Forkheadtranscription factors in the 5’ upstream regions of VMA genes (http://yetfasco.ccbr.utoronto.ca). (.pptx)

  • Figure S5 -

    Additional images of aging cells expressing endogenous GFP C-terminal fusions to the FKH1, FKH2 and HCM1 genes. (.pptx)

  • File S1 -

    Contains Tables S1 - S5. (.pdf)

  View article

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The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.

Online ISSN: 2160-1836

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