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Regulation of Gene Expression in Neurospora crassa with a Copper Responsive Promoter

Teresa M. Lamb, Justin Vickery and Deborah Bell-Pedersen
G3: Genes, Genomes, Genetics October 2013, g3.113.008821; DOI: https://doi.org/10.1534/g3.113.008821

Supporting Information for Lamb, Vickery, and Bell-Pedersen, 2013

Files in this Data Supplement:

  • Supporting Information - Figures S1-S6 and Tables S1-S2 (PDF, 865 KB)
  • Figure S1 - Copper levels control expression of the N. crassa high affinity copper transporter (tcu-1) gene in a WT strain. (PDF, 437 KB)
  • Figure S2 - Quantitation of tcu-1 expression after induction by BCS. (PDF, 524 KB)
  • Figure S3 - Integration of Ptcu-1 into the wc-1 locus. (PDF, 429 KB)
  • Figure S4 - Effects of glucose concentration on Ptcu-1 driven WC-1 protein. (PDF, 459 KB)
  • Figure S5 - Effects of copper and BCS on growth. (PDF, 410 KB)
  • Figure S6 - Effects of copper and BCS on Ptcu-1 driven HPT1-FLAG protein production. (PDF, 410 KB)
  • Table S1 - Primers used for Ptcu-1wc-1 strain construction. (PDF, 396 KB)
  • Table S2 - Primers used for Ptcu-1hpt-1 strain construction. (PDF, 395 KB)

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