Overexpression of miRNA-9 Generates Muscle Hypercontraction Through Translational Repression of Troponin-T in Drosophila melanogaster Indirect Flight Muscles

MicroRNAs (miRNAs) are small noncoding endogenous RNAs, typically 21–23 nucleotides long, that regulate gene expression, usually post-transcriptionally, by binding to the 3′-UTR of target mRNA, thus blocking translation. The expression of several miRNAs is significantly altered during cardiac hypertrophy, myocardial ischemia, fibrosis, heart failure, and other cardiac myopathies. Recent studies have implicated miRNA-9 (miR-9) in myocardial hypertrophy. However, a detailed mechanism remains obscure. In this study, we have addressed the roles of miR-9 in muscle development and function using a genetically tractable model system, the indirect flight muscles (IFMs) of Drosophila melanogaster. Bioinformatics analysis identified 135 potential miR-9a targets, of which 27 genes were associated with Drosophila muscle development. Troponin-T (TnT) was identified as major structural gene target of miR-9a. We show that flies overexpressing miR-9a in the IFMs have abnormal wing position and are flightless. These flies also exhibit a loss of muscle integrity and sarcomeric organization causing an abnormal muscle condition known as “hypercontraction.” Additionally, miR-9a overexpression resulted in the reduction of TnT protein levels while transcript levels were unaffected. Furthermore, muscle abnormalities associated with miR-9a overexpression were completely rescued by overexpression of TnT transgenes which lacked the miR-9a binding site. These findings indicate that miR-9a interacts with the 3′-UTR of the TnT mRNA and downregulates the TnT protein levels by translational repression. The reduction in TnT levels leads to a cooperative downregulation of other thin filament structural proteins. Our findings have implications for understanding the cellular pathophysiology of cardiomyopathies associated with miR-9 overexpression.

the appropriate stoichiometric balances, many levels of regulation are required. While transcriptional regulation of these components is extremely important, the significance of the roles of microRNAs (miRNAs) in myopathies in general, and in hypertrophy in particular, are becoming increasingly recognized (Eisenberg and Psaty 2009;Parkes et al. 2015).
miRNAs are small noncoding endogenous RNAs, typically 22-30 nucleotide long, that exert subtle control over gene expression, transcriptionally or post-transcriptionally. This makes them an indispensable part of the regulatory network in almost all complex events in organisms ranging from plants to mammals, and even their viruses (Bartel and Chen 2004;Allen and Howell 2010;Carthew and Sontheimer 2009). In muscles, many miRNAs-including both the muscle-specific "myomiRs" such as miR-1, miR-133, and miR-206 (McCarthy 2008), as well as those more widely expressed, such as miR-24, miR-29, and miR-181 (Erriquez et al. 2013)-are involved in the regulation of both myoblast proliferation and differentiation (Chen et al. 2008). Importantly, the expression of several miRNAs is significantly altered during cardiac hypertrophy, myocardial ischemia, fibrosis, heart failure, and other cardiac myopathies (Latronico and Condorelli 2011;Oliveira-Carvalho et al. 2012). miR-9, a miRNA of recognized neural functions (Gladka et al. 2012;Krichevsky et al. 2003), reportedly plays a regulatory role in myocardial hypertrophy and is antagonistic to myocardin, a positive mediator of cardiac hypertrophy (Wang et al. 2010). In addition, miR-9 was implicated in the regulation of platelet-derived growth factor receptor-b, a regulator of cardiomyocyte angiogenesis (Zhang et al. 2011). Both studies reported downregulation of miR-9 upon activation of the hypertrophic response. Recently, clinical studies revealed that miR-9 expression levels are significantly lower in hypertensive patients as compared to healthy controls, and appear to be correlated with ventricular mass (Kontaraki et al. 2014). Thus, miR-9 appears to have more than one role in cardiac function. It is thus imperative to characterize its roles in the context of muscle development and function. There is high evolutionary conservation of the ultrastructure of striated muscles, their component proteins, and the mechanisms that regulate the assembly of sarcomeres and myofibril formation throughout vertebrates; similarly, there is substantial similarity between the muscles of invertebrates with complex locomotion (Taylor 2006). The Drosophila IFMs provide a good system with which to study muscle development, function, and associated diseases (Sparrow et al. 2008). This is particularly true with respect to the investigation of cardiomyopathic disorders, as the IFMs exhibit properties such as stretch activation and asynchronous contraction that are physiologically similar to those of cardiac muscles (Vigoreaux 2001). Specific mutations of the Drosophila contractile machinery (Kronert et al. 1995;Nongthomba et al. 2003), signaling cascades (Gajewski et al. 2006), and connective tissues (Pronovost et al. 2013) lead to muscle hypercontraction, associated with decreased structural integrity of the sarcomeres, which is similar to that seen in many myopathic conditions of higher organisms, including humans. In particular, the Drosophila miR-9a is an exact copy of the human miR-9 (Yuva-Aydemir et al. 2011).
In this study, we have investigated the regulatory role of miR-9a in Drosophila for IFM development and functioning. We show that Drosophila miR-9a plays a novel role in the regulation of TnT, a major structural protein, during myofibrillogenesis. This finding will lead to a better understanding of how human miR-9 may be involved in the pathogenesis of cardiac hypertrophy.
Generation of UAS-TnT lacking the miR-9 binding site Two transgenic fly lines [UAS-TnT (10a) and UAS-TnT (10b)] were generated for the overexpression of either the adult isoform (10a) or the pupal isoform (TnT-10b). The TnT transcripts (both 10a and 10b) were amplified using cDNA extracted from wild-type thoraces, using primers designed to target the 59-and 39-UTRs but to exclude the miR-9a binding site. The primers were also modified to incorporate EcoRI and KpnI restriction sites for subcloning into the pUAST overexpression vector (TnT FP with EcoRI site: 59-GAACCGCAGAATTCGCTCC TAC-39, TnT RP with KpnI site: 59-GTGAAGGAAAGTGGTACCC GAG-39). The transcripts were cloned into a TA vector and the clones were screened for the presence of 10a or 10b transcripts using reverse primers specific for the alternatively spliced exon (TnT FP + TnT10a RP: 59-TTGTGCGCTGAGTGAATC-39 and TnT FP + TnT10b RP: 59-CGGTGTATTGCTCCTTCT-39). The presence of the 10a or 10b transcripts in the respective clones was confirmed by sequencing. The sequenced clones and pUAST plasmid were digested with EcoRI and KpnI, the released inserts and cut pUAST vector were ligated, and the transgenic constructs were cloned and confirmed by sequencing. The construct, either pUAST-TnT-10a or pUAST-TnT-10b, along with л-helper plasmid (encoding for a transposase) was injected into embryos of white-eyed w 1118 flies using the Olympus CK-X31-Narishige IM-9B microinjection system. The adult flies (G 0 ) that emerged were then crossed with white-eyed flies to produce the F 1 generation. The transgenic flies were identified by their red eye phenotype.

Behavioral test
Flight ability was assayed using 2-3-d-old individual flies as described previously (Drummond et al. 1991) and the flies were categorized as up-flighted, horizontally-flighted, down-flighted, or flightless. Each fly was flight tested three times.

Polarized microscopy
For polarized microscopy, 2-3-d-old flies were bisected and processed using a protocol described previously (Nongthomba and Ramachandra 1999). Images were captured using an Olympus SZX12 microscope fitted with an Olympus C -5060 camera.
Hematoxylin and eosin staining Hematoxylin and eosin staining of transverse sections of the adult thorax was done as previously described (Pantoja et al. 2013). Sections were mounted using Dibutylphthalate Polystyrene Xylene (DPX) mounting medium (Qualigens, Mumbai) and analyzed by light microscopy. Images were acquired using a Leica DFC300FX camera and processed using inbuilt software.

Confocal microscopy
The bisected fly hemithoraces were processed for immunohistochemical analysis as described previously (Rai et al. 2014). Following blocking, samples were treated differently depending on the type of analysis required.
Scanning electron microscopy (SEM) SEM analysis of IFMs was used to visualize sarcomeric structure. Threed-old flies were dehydrated using an alcohol series (50, 70, 80, 90, 95, and 100%). Samples were incubated for 10 min in each dilution with the final dehydration step in 100% alcohol repeated twice. After twice incubating each sample in hexamethyldisilazane for 45 min, they were dried in a desiccator for 24 hr. The head, wings, abdomen, and legs were then removed, transferred to a glass slide, and bisected sagittally. Bisected thoraces were mounted onto an aluminum stub with carbon tape and surface coated by gold sputtering (20 nm thick film) using a Baltec sputter to avoid charging. A Zeiss, Ultra 55, Field Emission SEM with Secondary Electron Detector was used for imaging with an accelerating voltage of 2-5 keV and 8 mm working distance.

Northern blotting
Detection of miRNA was carried out using the northern blotting protocol of Varallyay et al. (2008). RNA was extracted as described earlier, and quantified using a NanoDrop 1000 spectrophotometer (Thermo Scientific). Equal concentrations of samples were loaded on the gel. RNA bands were visualized under a UV transilluminator (JH BIO Innovations Pvt. Ltd.) and transferred onto a Nitrocellulose membrane (Millipore) by semidry transfer. Locked nucleic acid (LNA) Probe for miR-9a (59-TCATACAGCTAGATAACCAAAGA-39) and control probe for U6snoRNA (59-GTCATCCTTGCGCAGGGGCCATGC-39) was labeled using 1 ml T4 polynucleotide kinase (PNK), 1 ml [g-32 P] ATP, and 2 ml PNK buffer, and the final volume was made up to 20 ml. Following incubation at 37°for 60 min, the probe was purified using a Sephadex G-50 column. The membrane was washed twice with 0.5· TBE (Tris Borate-EDTA), allowed to cross-link under UV light for 60 sec, and then incubated at 40°for hybridization with LNA probe in 1· Perfect Hyb Plus buffer (Sigma) for 2-3 hr. The probed membrane was then washed three times with 2· SSC, 0.1% SDS buffer at 40°and exposed to photographic film. The exposed film was scanned using a Phosphor image scanner (GE Typhoon 9500).

Data availability
We have provided the details of all the web addresses of data resources that we made use of in this study. Other data that support our findings have been included as Supplemental Material and are described in the Results section. Supplemental Figure legends are available in File S1.

RESULTS
Knockdown of miR-9a in the IFMs during myofibrillogenesis does not affect muscle structure and function To investigate the role of miR-9a during myofibrillogenesis, we performed IFM-specific knockdown of miR-9a from 36 hr APF. Sarcomeres of the IFMs are established by an organized assembly of their structural proteins between 37 and 46 hr APF (Reedy and Beall 1993;Nongthomba et al. 2004). miR-9a is expressed in developing IFMs and the muscle attachment sites (Yatsenko and Shcherbata 2014), and expression is highly reduced in adult IFMs (Supplemental Material, Figure S1A). The knockdown of miR-9a during myofibril assembly had no detrimental effect on flight ( Figure S1B). Flies with knockdown of miR-9a (UH3 . miR-SP-9a) exhibited close to normal flight ability, with 74.2% of flies capable of upward flight and 25.8% exhibiting horizontal flight (n = 31), which is similar to wild-type, where 80.6% of flies were up-flighted and 19.4% were horizontally-flighted (n = 31) ( Figure S1B). Further, both wild-type ( Figure S1C) and miR-9a knocked-down flies ( Figure S1D) had six normal dorsal longitudinal muscles (DLM) fibers in each hemithorax with normal sarcomeric structures ( Figure S1, C'-D'').
Overexpression of miR-9a in the IFMs during myofibrillogenesis causes hypercontraction To determine whether the inherently low expression of miR-9a during myofibrillogenesis ( Figure S1A) is important for the critical roles of its targets during this stage of muscle development, we overexpressed miR-9a in the IFMs throughout myofibrillogenesis. While miR-9a expression was barely detectable in wild-type IFMs, UH3-Gal4-mediated (Singh et al. 2014) miR-9a overexpression clearly increased miR-9a levels ( Figure S1A) and adversely affected both wing posture and flight performance (Figure 1, A-B' and E).
The loss of muscle integrity and sarcomeric structure caused by miR-9a overexpression (Figure 1, D-D'') is very similar to the hypercontraction phenotype reported earlier (Nongthomba et al. 2003). This phenotype is usually associated with mutations in genes encoding sarcomeric structural proteins. It is well established in Drosophila IFMs that mutation in some of the structural proteins like TnT, Actin, and TnI can lead a the coordinated reduction in other thin filament proteins. These result in loss of sarcomeric structure and muscle hypercontraction, as a consequence of mis-regulated acto-myosin interaction (Nongthomba et al. 2003(Nongthomba et al. , 2004(Nongthomba et al. , 2007Firdaus et al. 2015). The hypercontraction phenotype can be suppressed in flies with the Mhc P401S mutation (Figure 1, G and G') (Nongthomba et al. 2003). This mutation is in the Actin binding head region of the myosin heavy chain, which prevents the interaction of the thin and thick filaments, thus reducing muscle contraction. Flies carrying Mhc P401S mutation in the miR-9a overexpression background showed suppression of the muscle structural defects associated with the miR-9a overexpression (Figure 1, F-H). The DLMs and sarcomeric structure in adult flies (Figure 1, G and G') were comparable to those of wild-type controls (Figure 1, F and F'). Compared to flies with overexpression of miR-9a alone (Figure 1, H and H'), the hypercontraction suppressed flies possessed six DLMs (asterisks) ( Figure 1G) with close to normal sarcomeric structures ( Figure 1G', white arrow shows a Z-disc), confirming that the miR-9a muscle phenotype results from unregulated acto-myosin interactions.
To confirm that the muscle defects observed resulted directly from miR-9a overexpression in the IFMs during myofibrillogenesis, we studied the effect of suppressing miR-9a expression in the overexpression background. Knockdown of miR-9a in the overexpression background rescued the flight ability to levels similar to wild-type flies ( Figure S1B). The adult flies had a normal arrangement and pattern of DLMs ( Figure  S2, D-E'). Thus, the hypercontraction was rescued and the IFMs showed ordered sarcomeres ( Figure S2, D-E') in contrast to those with the phenotype from overexpression of miR-9a alone (Figure 1, D and D').
Overexpression of miR-9a results in downregulation of TnT Hypercontraction results from the sarcomeres being unable to withstand the forces produced within them due to changes in the structural proteins of the sarcomere and/or their regulation. Therefore, we in-vestigated if any such proteins are targets of miR-9a. Bioinformatics analysis identified 135 potential miR-9a targets that were then functionally annotated. Five different prediction programs were used (see Materials and Methods) and we chose only those predicted targets that were detected by three or more programs. Functional annotation revealed that these genes are involved in a variety of cellular and developmental functions such as transcriptional regulation, protein degradation, apoptosis, endocytosis, neuronal specification, imaginal disc development, etc., but that 29 genes are involved in muscle development ( Figure 2A). All the putative miR-9a targets associated with muscle development proved to be involved in muscle function. Of these miR-9a targets in muscles, 4 genes are reported to be involved in larval muscle development, 19 in the development of pupal and adult muscles (Figure 2A) (Schnorrer et al. 2010), and 6 genes are associated with muscle development (Madan et al. 2017; https://www. ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70252). These putative targets are expressed in the IFMs as demonstrated using the Drosophila IFM microarray (https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE70252) ( Figure 2B).
Among the putative targets of miR-9a genes involved in muscle development and function is the up gene, which codes for all the TnT isoforms in Drosophila melanogaster. up showed a very high level of expression in adult IFMs (blue bar in Figure 2B, expression validated by real-time PCR in Figure 2C). The 39-UTR of the up gene has the miR-9 binding site sequence ( Figure 2D). Given that up mutations have been implicated in muscle hypercontraction (Nongthomba et al. 2003(Nongthomba et al. , 2007, we investigated whether miR-9a can cause the downregulation of TnT, possibly through binding to its target sequence at the 39-UTR of TnT mRNA and suppressing the translational process as represented in Figure 2D. Indeed, there was a significant reduction in TnT protein levels (P value , 0.002) in the DLMs of flies overexpressing miR-9a compared to wild-type ( Figure 2E). There was also a concomitant decrease in the levels of other structural proteins (which are not targets of miR-9a) that are part of the thin filament (Actin), including the Tn complex (TnI) ( Figure S3A). On the other hand, Flightin, a thick filament component, was not reduced in flies overexpressing miR-9a compared to wild-type flies ( Figure S3A). These results were expected since reduction of one thin filament protein is known to lead to a coordinated reduction of other thin filament proteins, but does not affect thick filament components (Nongthomba et al. 2004(Nongthomba et al. , 2007.

Repression of TnT by miR-9a is responsible for the hypercontraction phenotype
Since several putative miR-9a targets are important during muscle development, we asked whether the hypercontraction phenotype resulted directly from the downregulation of TnT by miR-9a or was due to the repression of other targets. First, we observed that the knockdown of TnT in IFMs during myofibrillogenesis generated a phenotype very similar to the overexpression of miR-9a. Knockdown of TnT resulted in the disruption of muscle structures ( Figure S3, E and E'), which was comparable to the muscle defects seen in overexpression of miR-9a ( Figure S3, F and F'). We further tested if the knockdown of some other predicted miR-9a targets can lead to similar defects in muscle structure and function. The downregulation of neuralized, an E3 ubiquitin ligase, failed to show any muscle defects ( Figure S3, C and C'). Flies with a knockdown of Sallimus (Sls) showed six DLMs ( Figure  S3D). However, reduction in Sls, which is a structural component of the Z-disc, did result in some tearing of the sarcomeres (rectangle, Figure  S3D'), but this was not comparable to the damage following miR-9a overexpression. To further confirm that that the muscle hypercontraction resulting from overexpressing miR-9a is a direct result of knockdown of TnT alone, we carried out a rescue of the muscle phenotype by overexpressing TnT devoid of the miR-9a binding site in flies with elevated levels of miR-9a in their IFMs. TnT transgenic lines were driven using UH3-Gal4 and shifted to 29°at 50-52 hr APF. Overexpression of transgenic TnT, either the TnT 10a or 10b isoform, devoid of the miR-9a binding sequence, restored TnT protein levels in these flies compared to flies overexpressing miR-9a alone ( Figure 3A). Importantly, the restoration of TnT levels in the background of miR-9a overexpression completely rescued the hypercontraction phenotype. This was evidenced by the presence of six DLMs ( Figure 3B') and the complete absence of any hypercontracted DLMs in the hemithoraces of these flies ( Figure 3C). Further, the flight ability of these flies was also partially rescued: control flies [(UH3/+; +; +) Gal4 flies] were 100% up-flighted (n = 31); for TnT 10a, 19.6% of flies were horizontally-flighted, 28.2% down-flighted, and 52.2% flightless (n = 46); and for TnT 10b: 4.8% flies were up-flighted, 37.1% horizontally-flighted, 22.6% down-flighted, and 35.5% were flightless (n = 62); as compared to the flies with overexpression of miR-9a alone, of which were all flightless (n = 60) ( Figure 3D). Both of these transgenic lines (TnT 10a and TnT 10b) showed almost complete restoration of muscle integrity and sarcomeric structure (Figure 4, B, B', D, and D') comparable to that of wild-type controls (Figure 4, A and A'), in stark contrast to the muscles in the flies overexpressing miR-9a (Figure 4, C and C').
These data argue that TnT is the major target of the miR-9a responsible for the muscle hypercontraction phenotype. We also confirmed that the rescue of the hypercontraction phenotype by expressing TnT isoforms in IFMs is indeed because of the restoration TnT and not due to Gal4 dilution. When UH3-Gal4 was used to drive both UAS-miR-9a and UAS-GFP, the progeny still exhibited hypercontracted muscles in hemithoraces with loss of sarcomeric structure ( Figure S4, B and B'), similar to the phenotype that results from driving UAS-miR-9a alone using UH3-Gal4 (Figure 1, D and D').

DISCUSSION
The present study throws light on a new role played by miR-9a during muscle development and function. Previously, Drosophila miR-9a has only been shown to be involved in neuronal differentiation, wing margin patterning, and myotendinous junction formation (Biryukova et al. 2009;Bejarano et al. 2010;Yatsenko and Shcherbata 2014). We have now shown that miR-9a is involved in the translational regulation of TnT levels during sarcomeric assembly.
TnT is a major target of miR-9a during myofibrillogenesis in the IFMs In general, miRNAs and their targets have been observed to exhibit mutually exclusive expression (Stark et al. 2005). While, miR-9a is strongly expressed in all developmental stages, its expression is reduced in adult flies, including the adult IFMs where its expression is much reduced compared to earlier developmental stages (Sempere et al. 2003). We have confirmed that miR-9a is barely detectable in adult IFMs ( Figure S1A). These data suggest that the expression of some miR-9a targets could be required for IFM development and function.
We report here that overexpression of miR-9a causes a hypercontraction phenotype in the IFMs. We identified TnT, a structural component of the thin filament of the sarcomere, as a major target for miR-9a in muscles, and have shown that miR-9a overexpression leads to repression of TnT and that this is sufficient to explain the IFM hypercontraction phenotype. In Drosophila, TnT is encoded by the up gene, is a key component in coordinating the Tn-Tm complex (Fyrberg et al. 1994;Nongthomba et al. 2007), and serves as an anchor for the other components of the complex which comprise TnI, TnC, and Tm (Gordon et al. 2000). Whereas the up 1 mutation is characterized by the absence of the adult IFM-specific TnT isoform, TnT-10a, resulting in hypercontraction (Nongthomba et al. 2007), the up 101 mutation leads to increased calcium sensitivity and irregular acto-myosin interactions, which also cause hypercontraction, producing damaged muscles (Beall and Fyrberg 1991;Nongthomba et al. 2003Nongthomba et al. , 2007. However, previously there has been no report that epigenetic regulation of TnT can also contribute to the maintenance of stoichiometric balance. To the best of our knowledge, this is the first report on the miRNA-mediated post-translational regulation of TnT.
Clearly, downregulation of TnT by miR-9a phenocopies the mutation in up via the same mechanism of stoichiometric imbalance that drives the mis-regulation of the acto-myosin interaction. Importantly, this demonstrates that not only defects in transcriptional control, but also the derailing of other regulatory processes such as miRNA-mediated control, can result in the same defects. Thus, our finding that miR-9a can alter levels of thin filament components via translational control of TnT demonstrates that miRNAs are not just "regulators of regulators," but can act as direct regulators in coordinating a complex process such as myofibrillar assembly. We show here that a major structural protein, TnT, can in fact be regulated by miRNA.
miR-9 is required for maintaining protein stoichiometry and may have implications in the etiology of myopathies Studies on IFM mutants indicate that structural integrity of IFMs is highly dependent on interactions between thin and thick filaments, as well as the ratio of individual myofibrillar contractile components. Any change in gene dosage and corresponding protein stoichiometry in the thin filaments translates into defects in normal myofibrillar assembly leading to hypercontraction (Tansey et al. 1991;Nongthomba et al. 2003Nongthomba et al. , 2007. This explains the myofibrillar defects that result from overexpression of TnT (Marco-Ferreres et al. 2005), Mhc (Cripps et al. 1994), and in most of the heterozygotes carrying mutations in genes encoding structural proteins (Prado et al. 1995;Gajewski and Saul 2010).
The phenomena of both hypercontraction and hypertrophy, although observably different, are both responses to the muscle contraction and the imbalance of structural proteins. In hypercontraction, the muscles show properly arranged sarcomeres during early development, but the subsequent uncontrolled acto-myosin interactions lead to stress and muscle tearing (Nongthomba et al. 2003). Hypertrophy, on the other hand, is characterized by an increase in muscle volume. For instance, myocardial hypertrophy is associated with cardiac remodeling where there is an increase of muscle wall thickness, but not through any increase in myocyte number (hyperplasia). However, cardiac hypertrophy is also a physiological response to stress induced by ischemia, mitochondrial defects, and mutations in sarcomeric components, etc. Importantly, mutations in the same gene orthologs that cause hypercontraction in Drosophila are the ones mutated in cardiac hypertrophy  patients as well. For example, mutations in the TnT gene are one of the predominant causes of hypertrophy (Seidman and Seidman 2001;Di Pasquale et al. 2012). Most of these TnT mutations exhibit increased calcium sensitivity and activation of muscle contractility (Harada and Potter 2004;Parvatiyar and Pinto 2015;Gilda et al. 2016), a similar pathogenesis to the hypercontraction produced by TnT mutants in Drosophila (Nongthomba et al. 2003(Nongthomba et al. , 2007. Viswanathan et al. (2014) have shown that the up 101 mutation generates a muscle abnormality similar to human cardiomyopathy through sensitive calcium regulation in the Drosophila heart.
Vertebrate TnT (TNNT) has a vital role in the anchoring of Tn-Tm to Actin and is also essential for Ca +2 -mediated activation and inhibition of acto-myosin activity during muscle contraction (Potter et al. 1995;Schiaffino and Reggiani 1996;Domingo et al. 1998;Perry 1998;Oliveira et al. 2000). Mutations in TNNT in Caenorhabditis elegans result in defects in embryonic body wall muscle contractions and sarcomere organization (Myers et al. 1996). Mutations in the cardiac isoform of TnT (TNNT2) are associated with familial HCM, dilated cardiomyopathy, or arthrogryposis (Thierfelder et al. 1994;Kamisago et al. 2000;Sehnert et al. 2002;Sung et al. 2003). TNNT2 was also found to be upregulated in cardiac hypertrophic or myocardial infarction conditions (Salic and De Windt 2012). However, there are still unanswered questions pertaining to the mechanisms by which cardiac TNNT2 upregulation is brought about during hypertrophy. Hence, the upstream players that regulate the level of cardiac TNNT2 during muscle development and function are very important. Drosophila miR-9a is identical to human miR-9 and the human TnT (TNNT) has significant homology to Drosophila TnT (Lagos-Quintana et al. 2001). Since miR-9a is capable of regulating TnT levels in Drosophila, it is possible that the human miR-9 may also play a role in regulating TNNT levels. Interestingly, sequence analysis of the human skeletal and cardiac isoforms of TNNT reveals that only the cardiac TNNT possesses the miR-9 binding site ( Figure S4C) while the skeletal isoforms lack it. Incidentally, bioinformatic analysis failed to find a miR-9 target site in the mRNA sequence of mouse TNNT. It is important to note that the initial report of miR-9's role in muscle hypertrophy were from studies on mice (Wang et al. 2010), so miR-9a could be playing varied roles in different organisms. Our study suggests that miR-9a might be involved in specifically regulating the levels of cardiac TnT in humans. It would be interesting to know if the increase in cardiac TNNT that occurs in response to hypertrophic stimulus is mediated by miR-9. Many mutations of the human cardiac TnT give rise to the hypertrophic condition (Di Pasquale et al. 2012). However, cardiac hypertrophy is a genetically and clinically heterogeneous disorder and its etiology in many instances has not been determined (Gilda et al. 2016). The present study provides a plausible candidate in the form of miR-9 to explore in the etiology of idiopathic cardiomyopathies. It would be interesting to determine what represses miR-9 during myofibril assembly. Its continuous expression would be deleterious to myofibril assembly through its repression of the expression of very important structural proteins such as TnT. Taurine-upregulated gene-1 (TUG1) negatively regulates miR-9 in a human cancer cell line (Zhao and Ren 2016). Whether TUG1 or similar protein(s) are involved in muscle hypertrophy/hypercontraction and myofibril assembly requires further investigation.