Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

Sonia El Mouridi; Claire Lecroisey; Philippe Tardy; Marine Mercier; Alice Leclercq-Blondel; Nora Zariohi; Thomas Boulin

doi:10.1534/g3.117.040824

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

View ORCID ProfileSonia El Mouridi, Claire Lecroisey, View ORCID ProfilePhilippe Tardy, View ORCID ProfileMarine Mercier, Alice Leclercq-Blondel, Nora Zariohi and View ORCID ProfileThomas Boulin

G3: Genes, Genomes, Genetics May 1, 2017 vol. 7 no. 5 1429-1437; https://doi.org/10.1534/g3.117.040824

Open Access

Sonia El Mouridi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Claire Lecroisey

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Philippe Tardy

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Marine Mercier

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Alice Leclercq-Blondel

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Nora Zariohi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Thomas Boulin

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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ORCID record for Thomas Boulin
For correspondence: thomas.boulin@univ-lyon1.fr

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Volume 7 Issue 5, May 2017

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Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

View ORCID ProfileSonia El Mouridi, Claire Lecroisey, View ORCID ProfilePhilippe Tardy, View ORCID ProfileMarine Mercier, Alice Leclercq-Blondel, Nora Zariohi and View ORCID ProfileThomas Boulin

G3: Genes, Genomes, Genetics May 1, 2017 vol. 7 no. 5 1429-1437; https://doi.org/10.1534/g3.117.040824

Open Access

Sonia El Mouridi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Claire Lecroisey

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Philippe Tardy

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Marine Mercier

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Alice Leclercq-Blondel

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Nora Zariohi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Thomas Boulin

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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For correspondence: thomas.boulin@univ-lyon1.fr

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Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

View ORCID ProfileSonia El Mouridi, Claire Lecroisey, View ORCID ProfilePhilippe Tardy, View ORCID ProfileMarine Mercier, Alice Leclercq-Blondel, Nora Zariohi and View ORCID ProfileThomas Boulin

G3: Genes, Genomes, Genetics May 1, 2017 vol. 7 no. 5 1429-1437; https://doi.org/10.1534/g3.117.040824

Open Access

Sonia El Mouridi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Claire Lecroisey

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Philippe Tardy

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Marine Mercier

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Alice Leclercq-Blondel

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Nora Zariohi

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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Thomas Boulin

Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69622 Villeurbanne, France

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ORCID record for Thomas Boulin
For correspondence: thomas.boulin@univ-lyon1.fr

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Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

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Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

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