Supplemental Material for Ho, et al, 2016

Files in this Data Supplement:

• File S1 - Illustrated protocol for mouse testis injection. (.docx, 1.01 MB)
• File S2 - Protocol for shRNA pool preparation and shRNA sequencing library preparation. (.docx, 43 KB)
• File S3 - Code for analyzing shRNA count data. (.zip, 5 KB)
• File S4 - Supplementary methods. (.docx, 154 KB)
• Table S1 - Summary of individual shRNA constructs used in this study. (.xlsx, 33 KB)
• Table S2 - Comparison of transfection rates. (.docx, 92 KB)
• Table S3 - Summary results of the pilot screen. (.txt, 4 KB)
• Table S4 - Summary results of the predicted genes screen. (.txt, 5 KB)
• Table S5 - shRNA read counts. (.xlsx, 151 KB)
• Figure S1 - Overview of shRNA library prep. (.jpg, 71 KB)
• Figure S2 - Standard curve for Actin and shRNA qPCR primers. (.png, 394 KB)
• Figure S3 - Functional networks for predicted genes. (.png, 1.34 MB)