A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

Brian H. Carrick; Linxuan Hao; Philip J. Smaldino; David R. Engelke

doi:10.1534/g3.115.025106

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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

Brian H. Carrick, Linxuan Hao, Philip J. Smaldino and David R. Engelke

G3: Genes, Genomes, Genetics March 1, 2016 vol. 6 no. 3 573-578; https://doi.org/10.1534/g3.115.025106

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Brian H. Carrick

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Linxuan Hao

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Philip J. Smaldino

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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David R. Engelke

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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For correspondence: david.engelke@ucdenver.edu

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Supporting Information for Carrick et al., 2016

Files in this Data Supplement:

Figure S1 - Plasmid map. (.pdf, 256 KB)
Figure S2 - CelTag fragment sequence with primer alignment. (.pdf, 561 KB)
Table S1 - Primer sequences. (.pdf, 64 KB)
File S1 - Complete plasmid sequence. (.pdf, 14 KB)

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Volume 6 Issue 3, March 2016

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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

Brian H. Carrick, Linxuan Hao, Philip J. Smaldino and David R. Engelke

G3: Genes, Genomes, Genetics March 1, 2016 vol. 6 no. 3 573-578; https://doi.org/10.1534/g3.115.025106

Open Access

Brian H. Carrick

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Linxuan Hao

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Philip J. Smaldino

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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David R. Engelke

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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For correspondence: david.engelke@ucdenver.edu

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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

Brian H. Carrick, Linxuan Hao, Philip J. Smaldino and David R. Engelke

G3: Genes, Genomes, Genetics March 1, 2016 vol. 6 no. 3 573-578; https://doi.org/10.1534/g3.115.025106

Open Access

Brian H. Carrick

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Linxuan Hao

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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Philip J. Smaldino

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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David R. Engelke

*Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, 48109-0600

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For correspondence: david.engelke@ucdenver.edu

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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

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Supporting Information for Carrick et al., 2016

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A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

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