Skip to main content
  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus
  • Other GSA Resources
    • Genetics Society of America
    • Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org
  • Log in
G3: Genes | Genomes | Genetics

Main menu

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • All Series
    • Genomic Prediction
    • Multiparental Populations
  • ARTICLE TYPES
    • About Article Types
    • Genome Reports
    • Meeting Reports
    • Mutant Screen Reports
    • Software and Data Resources
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Email alerts
    • RSS feeds
  • Other GSA Resources
    • Genetics Society of America
    • Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org

User menu

Search

  • Advanced search
G3: Genes | Genomes | Genetics

Advanced Search

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • All Series
    • Genomic Prediction
    • Multiparental Populations
  • ARTICLE TYPES
    • About Article Types
    • Genome Reports
    • Meeting Reports
    • Mutant Screen Reports
    • Software and Data Resources
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Email alerts
    • RSS feeds
Previous ArticleNext Article

A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel

View ORCID ProfileLaura K. Hilton, Fabian Meili, Paul D. Buckoll, Julie C. Rodriguez-Pike, Courtney P. Choutka, Jaime A. Kirschner, Freda Warner, Mette Lethan, Fabian A. Garces, Jingnan Qi and View ORCID ProfileLynne M. Quarmby
G3: Genes, Genomes, Genetics October 1, 2016 vol. 6 no. 10 3409-3418; https://doi.org/10.1534/g3.116.034264
Laura K. Hilton
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Laura K. Hilton
Fabian Meili
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul D. Buckoll
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie C. Rodriguez-Pike
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Courtney P. Choutka
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaime A. Kirschner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Freda Warner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mette Lethan
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabian A. Garces
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingnan Qi
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lynne M. Quarmby
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lynne M. Quarmby
  • For correspondence: quarmby@sfu.ca
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
Loading

Abstract

With rare exception, ciliated cells entering mitosis lose their cilia, thereby freeing basal bodies to serve as centrosomes in the formation of high-fidelity mitotic spindles. Cilia can be lost by shedding or disassembly, but either way, it appears that the final release may be via a coordinated severing of the nine axonemal outer doublet microtubules linking the basal body to the ciliary transition zone. Little is known about the mechanism or regulation of this important process. The stress-induced deflagellation response of Chlamydomonas provides a basis to identifying key players in axonemal severing. In an earlier screen we uncovered multiple alleles for each of three deflagellation genes, ADF1, FA1, and FA2. Products of the two FA genes localize to the site of axonemal severing and encode a scaffolding protein and a member of the NIMA-related family of ciliary-cell cycle kinases. The identity of the ADF1 gene remained elusive. Here, we report a new screen using a mutagenesis that yields point mutations in Chlamydomonas, an enhanced screening methodology, and whole genome sequencing. We isolated numerous new alleles of the three known genes, and one or two alleles each of at least four new genes. We identify ADF1 as a TRP ion channel, which we suggest may reside at the flagellar transition zone.

  • calcium
  • Chlamydomonas reinhardtii
  • TRP15
  • FAP16
  • Mutant Screen Report

During interphase, basal bodies nucleate the axonemal core of cilia. Although cilia perform a variety of important functions (Quarmby and Leroux 2010; Nachury 2014; Wang and Barr 2016; Morgan and Birman 2016; Hilgendorf et al. 2016), they are almost always lost when cells enter mitosis and basal bodies resume their roles as centrosomes (Quarmby and Parker 2005; Kim and Tsiokas 2011; Ke and Yang 2014; Cross and Umen 2015). Cilia are also shed in response to stress, a process known as deflagellation (Craige et al. 2013; Marshall 2008; Quarmby 2004). Physiological experiments with the unicellular alga Chlamydomonas reinhardtii reveal that axonemal severing is triggered by an influx of Ca2+ near the base of the flagella (Quarmby and Hartzell 1994; Quarmby 1996; Wheeler et al. 2008). In Chlamydomonas, the final event in premitotic ciliary disassembly is axonemal severing at the transition zone (TZ) between the cilium and basal body, similar to the severing that occurs during deflagellation (Parker et al. 2010; O’Toole and Dutcher 2014).

In a previous screen for deflagellation-defective mutants, we used insertional mutagenesis to tag affected genes for subsequent cloning and identification (Finst et al. 1998). We uncovered 13 mutant strains that resolved into multiple alleles of each of three deflagellation genes: FA1, FA2, and ADF1 (Finst et al. 1998). Adf mutants deflagellate if permeabilized with detergent or dibucaine in the presence of Ca2+, indicating that they have functional axonemal severing but are defective in the pathway that activates Ca2+ influx (Quarmby and Hartzell 1994; Quarmby 1996). In contrast, fa mutants fail to deflagellate even when treated with detergent or dibucaine, indicating defective axonemal severing (Lohret et al. 1998). The identity of ADF1 remained elusive, but we identified FA1 as a low complexity scaffolding protein (Finst et al. 2000), and FA2 as a NIMA-related kinase (Mahjoub et al. 2002). As a Nek kinase, FA2 belongs to an ancient and diverse clade of kinases with dual roles in cilia and cell cycle regulation (Parker et al. 2007). FA2 localizes to the TZ both in Chlamydomonas cells and when expressed in mammalian cells (Mahjoub et al. 2004), providing evidence of a conserved pathway for activating axonemal severing.

We propose that some genes involved in severing the axoneme during deflagellation and preceding mitosis are shared. Thus, although deflagellation itself is not essential to life, genes encoding proteins involved in both processes would be essential. Therefore, the identification of conditional mutants could deepen our understanding of an important premitotic activity.

The advent of whole genome sequencing (WGS) opened the door to identifying conditional mutations in Chlamydomonas (Lin and Dutcher 2015). Knowing that we had missed at least two activities (microtubule severing and calcium sensing) in our original screen, we conducted an unbiased forward genetic screen for conditional deflagellation mutants. We report identification of ADF1, discovery of at least four new deflagellation loci, and identification of flagellar protein FAP16 as a player in the signaling pathway.

Materials and Methods

Strains and culture conditions

The wild-type 137c mt+ (CC-125) and S1D2 mt− strains (CC-2290) were obtained from the Chlamydomonas Resource Center (University of Minnesota). The Chlamydomonas bacterial artificial chromosome (BAC) library was from Clemson University Genomics. Cells were maintained on solid tris-acetate-phosphate (TAP; Harris 2009) plates containing 1.5% agar at 21° and under constant illumination. The adf1, fa1, and fa2 strains isolated in our previous genetic screen (Finst et al. 1998) are also available from the Chlamydomonas Resource Center. To induce gametogenesis for mating, cells were maintained on low-N TAP media for 24–48 hr.

Genetic screen

To mutagenize, 5 × 106 wild-type cells in 20 ml of TAP were irradiated with ultraviolet light for 3 min at 254 nm, then left in the dark for 24 hr to prevent photo-reactivation. They were then plated onto 1.5% agar TAP plates at low density to obtain single colonies. After 4-6 d in continuous light at 21°, individual colonies were picked to agar plates for further growth. To test for deflagellation phenotype, cells were inoculated in 100 µl liquid TAP in 96-well plates and incubated at 21° for 2 d, then transferred to the restrictive temperature of 33° for 6 hr. For manual screening, colonies were screened for acid deflagellation by light microscopy (Finst et al. 1998). To make the screening process faster, we introduced a plate reader screen strategy based on Engel et al. (2011); absorbance at 510 nm was measured to establish the baseline density of motile cells before treating wells with 1.25 vol of Acid Deflagellation Buffer (ADB; 40 mM sodium acetate, pH 4.5, 1 mM CaCl2; Finst et al. 1998) for 45 sec, followed by neutralization with 1 vol of 0.1 M NaHCO3. Cells were then left to phototax above a light source for 30 min, after which another absorbance reading was taken. The change in absorbance was calculated as follows:Embedded Imagewhere Embedded Image is absorbance before deflagellation and Embedded Image is absorbance after deflagellation and phototaxis. Each plate had two wells of each of 137c mt+ (fully deflagellated) and an established adf-mutant (adf1-15 E20 G2), and the absorbance readings from these controls were used to establish a cutoff Embedded Image for each plate screened to flag colonies for further deflagellation assays. Potential mutants were then rescreened by light microscope manually, as described above. Of the 46,500 total colonies screened for deflagellation, 27,500 were screened using the plate method. The rate of recovery of mutants in the manual screen was about one per 1000 colonies screened, whereas it was only 0.6 per 1000 colonies screened for the plate method. Notably, the plate screening method was sensitive enough to detect a weak conditional mutant, adf4-1.

Mutant classification

In order to classify all mutant isolates as either adf or fa, cells in liquid culture were treated with 1.25 vol ADB or 1 vol of 150 µM dibucaine on a slide, and fixed with 1 vol of 3% glutaraldehyde after 45 sec. A separate sample of cells was fixed directly in 1 vol of glutaraldehyde to control for the fraction of flagellated cells before deflagellation. To classify mutants as temperature sensitive (ts), cultures of cells were divided and half were incubated at 33° for 6 hr and the other half remained at 21°. Percentage deflagellation was calculated as follows (Finst et al. 1998):Embedded Imagewhere BFC is the number of biflagellate cells per 100 cells, c is control, and e is experimental.

PCR-based recombination mapping

To map the causative mutations in each of our new adf mutants, we crossed each strain with the polymorphic mapping strain S1D2 (Silflow et al. 1995), and used primers from the Kit for Molecular Mapping of Chlamydomonas Genes version 1.1 (Chlamydomonas Resource Center, University of Minnesota; Rymarquis et al. 2005) for PCR-based recombination mapping. Detailed recombination mapping of adf1 was performed by crossing adf1;pf16 mutants with S1D2, and identifying recombination events in single mutant progeny using custom primers throughout the ADF1 region (Kirschner 2009).

WGS

Genomic DNA was isolated by two cycles of chloroform extraction and ethanol precipitation (Kirschner 2009). For some mutant strains (identified in Supplemental Material, Table S1), at least 5 µg of DNA was submitted to Genewiz (www.genewiz.com) for library preparation and sequencing on a HiSequation 2500 platform in a 2 × 100 bp paired-end (PE) configuration (Table S1). For other strains, genomic DNA libraries were prepared as follows: 5 µg of genomic DNA in 50 µl dH2O was fragmented on a Covaris M220 ultrasonicator, using the manufacturer’s 800-bp fragment size protocol. The fragments were dual size selected with a 0.4 vol ratio of beads followed by a 1.0 vol ratio of beads. Fragmented DNA was then ligated to adapters and indexed using the NEBNext Ultra DNA Library Prep Reagent Set for Illumina, as per the manufacturer’s protocol. We monitored DNA fragment size and library concentration with Agilent Bioanalyzer 2100 and Qubit fluorometer (Life Technologies), respectively. For adf2-1 and adf5-1, 0.05 pmol was run on a MiSeq platform using the 300-cycle (150 bp PE) V2 reagent kit. The remaining libraries were sequenced at The Center for Applied Genomics (Hospital for Sick Children, Toronto) on a HiSequation 2500 platform in a 2 × 125 bp PE configuration (Table S1).

The MiSeq and HiSeq outputs were processed as follows: The reads were aligned to the reference genome (C. reinhardtii v5.0 assembly; Merchant et al. 2007) using Bowtie2 and BWA-MEM, and each analyzed for single nucleotide variants (SNVs) and indels via the Genome Analysis Toolkit (GATK) Haplotypecaller and samtools mpileup (McKenna et al. 2010; Li and Durbin 2009; Langmead 2010; Li et al. 2009; Li 2011). All four variant (vcf) files were then combined into one, and analyzed for overlapping variant calls. Each vcf file was subtracted against vcf files from other mutant strains to remove parental variants. The BWA-MEM–generated alignments for all adf mutants were also analyzed with the variant caller Strelka (Saunders et al. 2012), to search for SNVs and small indels, using the sequence from fa1-6 as the normal sample for subtraction. Variant calls were filtered for homozygous mutations using SnpSift (Cingolani et al. 2012a), then annotated with SnpEff (Cingolani et al. 2012b) to predict the impact of variants (stop codons, splice site mutations, amino acid substitutions, synonymous mutations, or UTR/intron mutations). The SnpEff reference database was built from the C. reinhardtii reference genome and genome annotation v5.5 (Creinhardtii_281_v5.5.gene.gff3.gz; Merchant et al. 2007).

To check for large indels and other structural variants, such as duplications, inversions, and translocations, the BWA-MEM alignment for all adf mutants were analyzed with the structural variant caller Manta (Chen et al. 2016), using the sequence from fa1-6 as the normal sample for subtraction. Resulting vcf files were input into the Integrated Genomics Viewer (Broad Institute), along with corresponding bam files, to examine the evidence for each predicted structural variant.

Chlamydomonas transformation

Qiagen Plasmid Midi and Maxi kits were used to prepare plasmid and BAC DNA, respectively. Chlamydomonas cells were transformed using the glass bead method (Kindle 1990). For BAC or digested BAC transformations, cells were cotransformed with plasmid pSI103, which confers resistance to paromomycin (Sizova et al. 2001). Paromomycin-resistant colonies were screened for rescue of the deflagellation defect, as described in Finst et al. (1998).

PCR confirmation of adf1-3 deletion and rescue

To confirm the putative deletion in adf1-3 detected by WGS, we designed one primer to anneal within the deletion (GGCCGACATCTACAGGTTGT) and one just downstream of it (GCCTCTCTCACTCCCCCTAC). This primer pair should produce a 2-kb product in strains lacking a deletion. As an internal control, we used primers that amplify IFT20 (forward: TGGACGCGGTAGATAGAGGA, reverse: TCACCTTGATCAGCGACTGC), which produces a 1-kb PCR product.

Phylogenetic analysis

For the TRP15 phylogenetic analysis, representative transient receptor potential (TRP) channel sequences were obtained for each TRP family and the Shaker K+ channel outgroup, according to Peng et al. (2015), and included Chlamydomonas TRP15, Shaker, and PKD2 sequences. Accession numbers are available in Table S2. Ion transport domains were identified by InterProScan (http://www.ebi.ac.uk/interpro) and aligned with MUSCLE (Edgar 2004). The phylogenetic tree was inferred using maximum likelihood, a WAG+F+G amino acid substitution model, and a bootstrap value of 100, using MEGA7 (Kumar et al. 2016).

The FAP16 phylogenetic analysis was completed using phylogeny.fr (Dereeper et al. 2008) as follows: Sequences were aligned with MUSCLE (Edgar 2004) and curated to remove gaps using G-blocks (Castresana 2000). The phylogenetic tree was created with PhyML using the WAG amino acid substitution model and an approximate likelihood ratio test (Guindon et al. 2010; Anisimova and Gascuel 2006). Trees were rendered using TreeDyn (Chevenet et al. 2006). Sequences were obtained by BLAST against human EML1. Accession numbers are available in Table S3.

Resource and data availability

All new mutant strains have been deposited at the Chlamydomonas Resource Center (University of Minnesota). WGS data are available at NCBI BioProject PRJNA338310.

Results and Discussion

Forward genetic screen for deflagellation mutants

Following ultraviolet mutagenesis, single colonies were isolated, grown in liquid media, and manually screened for deflagellation defects in response to pH shock, as described in Finst et al. (1998). From 46,500 total colonies screened, we uncovered 36 new strains defective in the deflagellation response. The new mutants were classified as adf or fa on the basis of their deflagellation response to pH shock or dibucaine. Mutants that deflagellate in response to dibucaine but fail to deflagellate in response to pH shock were classified as adf, while mutants that fail to deflagellate in response to either treatment were classified as fa. Populations of mutants were frequently not uniform in their deflagellation response. Strains that yielded a higher proportion of deflagellating cells at 21° than 33° were categorized as ts. Of the 36 new deflagellation-defective strains, 30 were classified as adf and six as fa.

Mapping of mutant strains

Prior to and in parallel with the screen, we did fine resolution genetic mapping of mutant adf1 alleles isolated in the screen by Finst et al. (1998). Four of the five original adf1 alleles were generated by insertional mutagenesis, yet none of them retained sequence tags, thereby frustrating identification of the causative mutation. From crosses between pf16;adf1-2 and the mapping strain S1D2, we identified three recombination events between adf1-2 and OEE1 out of 920 meioses examined. These data indicate that ADF1 resides 0.33 map units from OEE1. To further define the genomic location of the ADF1 gene, we performed extensive PCR-based recombination mapping using custom-designed primers (Kirschner 2009). This work located ADF1 to a 380 kb region containing 42 predicted genes (Figure 1A).

Figure 1
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1

Identification of the ADF1 gene. (A). A schematic depicting gene predictions within the genomic region known to contain the ADF1 gene on the basis of recombination mapping. Shown below are the boundaries of BACs spanning this region. (B) A schematic of the genomic region covered by BAC 24I14, which complements the adf1 mutation, showing gene predictions and EcoRI restriction sites (black lines). (C). Deflagellation responses of WT, adf1-3, and the strain of adf1-3 rescued with EcoRI-digested BAC 24I14 (adf1-3R). Mean of three independent experiments ± SD.

We selected 17 of the 30 new adf mutants for PCR-based mapping using markers from the C. reinhardtii mapping kit (Table 1; Rymarquis et al. 2005). Eleven mutants showed linkage to the marker PSBO on chromosome 9 and are hypothesized to be alleles of adf1. Other strains were assigned adf gene numbers on the basis of linkage to markers on distant regions of the genome. The ts adf mutant E4N23 is linked to marker CNA34 on chromosome 3, and is now known as adf2-1; additional mapping with custom primers refined this location (Rodriguez Pike 2013). Two strains, E3.1 P15 B4 (adf3-1) and E21 C7 (adf3-2), are linked to marker TUG on chromosome 6. We hypothesized (and confirm below) that these strains are alleles of the gene we are calling ADF3. The mutation in E4.1 P3 H3 (adf4-1) is linked to marker PPX1 on chromosome 9 (see Table 1). We could not establish linkage between the mutant phenotype and any of the mapping kit markers for two of the adf strains (E4.1 P1 H3 and E5.1 P8 A12).

View this table:
  • View inline
  • View popup
Table 1 Results of recombination mapping of new adf mutant isolates, indicating the recombination frequency between the mutation and the closest mapping kit marker

New fa strains were crossed with known fa alleles fa1-4 and fa2-2, and the progeny screened for deflagellation. The data in Table 2 suggest that we isolated two new alleles of fa1 and four new alleles of fa2. WGS of several alleles confirmed the presence of mutations in the new alleles of fa1 and fa2 (Table 2). Therefore, we did not identify any new FA genes through this screen.

View this table:
  • View inline
  • View popup
Table 2 Identification of new fa strains as alleles of fa1 and fa2

Identification of causal mutations by WGS

We performed WGS on 29 strains, including five original adf1 alleles. We used three different WGS library prep and sequencing strategies: 1) Illumina MiSequation 150 bp PE sequencing using NEBNext Ultra library prep, 2) HiSequation 101 bp PE sequencing prepped in a commercial sequencing lab, or 3) HiSequation 126 bp PE sequencing using NEBNext Ultra library prep.

The MiSeq and HiSeq outputs were processed as follows: Reads were aligned to the reference genome (C. reinhardtii v5.0 assembly) and analyzed for SNVs and insertions and deletions (indels) that lay within exons and intron-exon boundaries (C. reinhardtii v5.5 annotation). Because the parental strain (CC-125) contained a large number of variants relative to the Chlamydomonas reference genome (Lin et al. 2013), the list of variants for each mutant strain was compared to two other unlinked mutant strains from our study, and identical variants were removed. In the strains sequenced by HiSeq, ∼20% of the variants are unique to each strain, while MiSeq identified more variants overall, and more unique variants (60–70%), likely due to lower coverage of the genome from MiSeq (Table S1). Lastly, to identify potential causative mutations, we cross-referenced our mapping data with sequencing data (Table 3).

View this table:
  • View inline
  • View popup
Table 3 Proposed causative mutations identified by WGS

The deflagellation defects in E4.1 P1 H3 and E5.1 P8 A12 are not linked to any of the standard mapping kit markers, thereby eliminating potential causative mutations close to any mapping kit markers. This left two candidate mutations for E4.1 P1 H3 and five candidates for E5.1 P8 A12. We designed custom PCR mapping markers for each of the candidate mutations and tested them for linkage to the deflagellation phenotype. None of the candidate mutations were linked to the deflagellation phenotype in either of these strains, thus E4.1 P1 H3 and E5.1 P8 A12 remain unmapped and unidentified.

ADF1 encodes TRP15, a TRP family Ca2+ channel

Of the 42 predicted genes in the known ADF1 region, 35 are covered by at least one BAC clone (Figure 1A). We transformed adf1-3 with various BAC clones or single-gene subclones from the known ADF1 region (Kirschner 2009; Rodriguez Pike 2013). At least 250 transformants of each of the candidate genes were assayed for complementation of the adf phenotype. Some of the BACs were digested with restriction enzymes prior to transformation so as to increase the likelihood that certain candidate genes would be incorporated intact (Cerutti et al. 1997).

Only one wild-type transformant was obtained, and it was generated by complementation with EcoR1-digested BAC 24I14 (PTQ8981), suggesting that ADF1 might be encoded by one of 11 genes that remained intact on this BAC (Figure 1, B and C). To rule out the possibility that this was a wild-type contaminant or a spontaneous reversion, we back-crossed the rescue strain with a wild-type strain and assayed for the deflagellation phenotype of the progeny. If this were a wild-type contaminant or reversion, then all of the progeny would be wild-type for deflagellation; if this was rescue or suppression, then a quarter of the progeny should exhibit the adf phenotype. Consistent with complementation, of the 56 random progeny assayed, 18 (32%) had the adf phenotype. Furthermore, crosses between the complemented adf1-3 strain (adf1-3R) and adf1-1 produced 115 random progeny, of which 64 (56%) were wild-type for deflagellation. Together, these data indicate that the BAC fragment restores wild-type deflagellation to at least two alleles of adf1.

Using WGS, we discovered that seven adf1 alleles (three from the 1998 collection and four from the new screen) all carry coding mutations in the same gene: TRP15, encoding a putative Ca2+ channel (Figure 2A and Table 3). All seven of these adf1 alleles have a deflagellation phenotype similar to that of adf1-2 (Figure 2B). Using the structural variant caller Strelka, we also identified a 1.9 Kb deletion in adf1-3, which deletes all of the 5′ UTR and the first 522 bp of the first exon of TRP15 (Figure 2A and Table 3). We confirmed this deletion by PCR, using a forward primer that anneals inside the putative deletion and one that anneals upstream of it. The deleted DNA was restored when adf1-3 was rescued by transformation with EcoR1-digested BAC 24I14 (Figure 2C). Importantly, the TRP15 gene is carried intact by an EcoR1 fragment of BAC 24I14 (Figure 1B). This supports the idea that restoration of the wild-type deflagellation phenotype of adf1-3 and adf1-1 was due to rescue and not suppression (Figure 1C).

Figure 2
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2

ADF1 encodes a TRP family ion channel, TRP15. (A) Schematic diagram of TRP15, showing mutation sites in indicated alleles along with the PFAM ion channel prediction. (B) Deflagellation phenotypes of the adf1 alleles. Each value is the mean of two or more independent experiments ± SEM. (C) PCR confirms the presence of a deletion in TRP15 in adf1-3, which is restored in adf1-3R. Of the TRP15 primers, one anneals within the deleted sequence and the other anneals just upstream of it. IFT20 was PCR-amplified in the same reaction as a positive control. (D) Phylogenetic analysis placing TRP15 in the context of metazoan TRP clades.

As described, both WGS and PCR confirm that the missing sequence is restored in the rescue strain, adf1-3R. Additionally, WGS coverage for the entire span of the EcoRI BAC fragment carrying TRP15 is 149.2-fold in adf1-3R vs. 70.0-fold in the flanking sequence, representing a full doubling of sequence coverage for that one region. In adf1-3, WGS coverage is equal for the region corresponding to the EcoRI fragment relative to the flanking sequence (56.4-fold vs. 54.0-fold). We conclude that mutations in TRP15 cause the deflagellation defect in adf1 mutants, and that adf1-3R is a bona fide rescue.

The TRP family is an ancient family of ion channels with diverse functions (Peng et al. 2015). We replicated the construction of the phylogenetic tree of TRP channels in Peng et al. (2015) and included TRP15 to determine its relationship to other members of the TRP channel family. Based on this analysis, we conclude that TRP15 does not align with any of the metazoan TRP channel clades (Figure 2D). The identification of ADF1 as an ion channel is gratifying, given our earlier data demonstrating that an influx of Ca2+ is required to trigger deflagellation (Quarmby and Hartzell 1994; Quarmby 1996).

ADF3 encodes the flagellar protein FAP16

Strains adf3-1 and adf3-2 have strong defects in acid-induced deflagellation, but are almost wild type in their response to dibucaine (Figure 3A). WGS identified mutations in FAP16 in both alleles (Table 3), 535 kb from the mapping kit marker TUG, which had 22% recombination with the deflagellation phenotype in adf3-2. To confirm this gene assignment, adf3-1 and adf3-2 cells were transformed with BAC 24K24 (PTQ9022), and we recovered one complemented strain with wild-type deflagellation for each of the two alleles (200 transformants screened for each strain; Figure 3B). In the region covered by BAC 24K24 (73 kb containing 10 predicted genes), WGS coverage for adf3-1 is 14.5-fold (obtained by MiSeq, Q-score ≥ 10), while coverage for adf3-2 is 35.1-fold (obtained by HiSeq Q-score ≥ 10). We manually examined the region covered by this BAC for insertions or deletions. In addition to the original analysis with samtools mpileup and GATK HaplotypeCaller, we looked for variants using Strelka and the structural variant caller Manta. We found no other variants of any kind in either of the alleles in the region covered by BAC 24K24, and FAP16 is the only gene in the region with variants in both adf3-1 and adf3-2. We conclude that ADF3 is FAP16.

Figure 3
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3

ADF3 encodes FAP16, a 192 kD flagellar protein. (A) Deflagellation of the newly discovered adf3-1 and adf3-2 in response to both dibucaine and acid at 21° and at 33°. Mean of two independent experiments ± SD. (B) Deflagellation response of the two new mutant strains, each transformed with BAC 24K24. Mean of four independent experiments ± SD. (C) Schematic diagram of FAP16 and the predicted truncated protein products of adf3-1 and adf3-2. (D) Phylogenetic tree placing FAP16 in the context of known EML proteins.

FAP16 is a protein of unknown function that was identified in the flagellar proteome of Chlamydomonas (Pazour et al. 2005). It encodes a 192 kD protein annotated with eight WD40 domains. In adf3-1, the causative mutation is a C > G transversion resulting in the loss of a splice site before exon 19; in adf3-2, the causative mutation is Y841*. These mutations are predicted to truncate the protein to 73 and 78 kD, respectively (Figure 3C).

WD40 domains are common among diverse proteins, serving generally as sites of protein–protein interactions (Stirnimann et al. 2010). BLAST analysis indicates that FAP16 is most similar to a family of microtubule-binding proteins, known as EMLs. Members of the EML family have been shown to be required for microtubule formation and have been implicated in ciliopathies (Lyman and Chetkovich 2015; Pollmann et al. 2006). Our phylogenetic analysis suggests that of the human EMLS, FAP16 is most closely related to EML5 and EML6 (Figure 3D). As a flagellar- and possibly microtubule-associated protein with a signaling (adf) rather than severing (fa) phenotype, FAP16 may serve to localize signaling proteins to the site of severing. The site of axonemal severing defines the juncture between the flagellum and the TZ. The deflagellation protein FA2 localizes to this site and a fraction of the FA2 leaves with the flagella when they are shed (Mahjoub et al. 2004). We speculate that FAP16 might similarly localize.

ADF2 and ADF4 remain unassigned

The adf2-1 strain is the only mutant we recovered with a ts deflagellation phenotype (Figure 4A). Wild-type cells deflagellate completely in response to pH shock or dibucaine, regardless of the temperature, and adf1-2 cells do not deflagellate in response to pH shock at either temperature. In contrast, adf2-1 mutant cells deflagellate much more in response to pH shock at the permissive temperature than at the restrictive temperature.

Figure 4
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4

The deflagellation phenotypes of adf2-1 and adf4-1 are conditional on temperature and nutrient status, respectively. (A) Deflagellation responses of the newly discovered adf2-1 strain, at both 21° and 33°. Mean of three independent experiments ± SD. (B) Deflagellation response of adf4-1 after 24 and 48 hr in low-N TAP media. Mean of four independent experiments ± SD.

We mapped the adf2-1 mutation to chromosome 3, but the combination of mapping and WGS (by MiSeq) did not provide sufficient resolution for us to assign the gene. One attractive candidate for the causal mutation is W71* in an inositol 1,3,4-trisphosphate 5/6 kinase (ITPK1; Cre03.g182100). In Arabidopsis, the related enzyme ITPK4 acts in the main pathway to IP6 (phytic acid) synthesis, impacting both signaling pathways and calcium storage (Kim and Tai 2011). Intriguingly, the knockdown of IPK1, the IP5-phosphorylating enzyme in zebrafish, reduces ciliary length and motility, linking a ciliary phenotype to a reduction in this inositol phospholipid kinase (Sarmah et al. 2007). Nevertheless, we cannot yet assign this gene as ADF2 because we have only isolated a single allele of adf2, and we have not shown tight linkage between the phenotype and this mutation.

In working with adf4-1 cultures, we observed that the deflagellation phenotype was often weak. Serendipitously, we discovered that cultures maintained in liquid TAP for long periods (≥5 d) had a stronger deflagellation-defective phenotype than freshly inoculated cultures. We hypothesized that nutrient starvation, possibly nitrogen starvation, was responsible for this effect. To test this, we inoculated adf4-1 and wild-type strains in liquid TAP medium for 48 hr, then treated them in one of three ways: fresh liquid TAP for 48 hr; fresh TAP for 24 hr followed by 24 hr in low-N tap; or low-N liquid TAP for 48 hr. The acid-deflagellation defect was strongest after 48 hr low-N liquid TAP (Figure 4B). Discovery of a nutrient-based conditionality is novel in the deflagellation pathway and it is yet to be determined whether this phenotype is related to gametogenesis.

We mapped adf4-1 to a region of chromosome 9 distinct from the ADF1 region (see Table 1). Examination of our WGS data in this region led us to identify a candidate for causal mutation: D780N missense mutation in Cre09.g386732, annotated as a glycosyltransferase. We refrain from assigning this as ADF4 for the following reasons: we have identified only the one allele, we have not established tight linkage between the mutation and the phenotype, and the D780 is not a conserved amino acid. Therefore, ADF4 remains unidentified.

Conclusions

We report at least five new deflagellation genes, map four of them, and identify two. ADF1 is revealed as a TRP channel and ADF3 as flagellar protein FAP16. We used genetic mapping and WGS to identify candidate mutations. Gene assignments were confirmed by complementation of the deflagellation phenotype with the wild-type gene.

Acid-induced deflagellation is triggered when weak organic acids freely permeate the cell membrane (Hartzell et al. 1993). Inside the cell, dissociation leads to acidification of the cytoplasm and localized Ca2+ influx near the base of the flagella (Quarmby 1996; Wheeler et al. 2008). We propose that TRP15 is the acid-activated entry route for calcium. In response to the Ca2+ signal, microtubules are severed at a precise site at the base of the flagella, distal to the flagellar TZ. We propose that FAP16 connects the signaling components to the microtubule-severing machinery. We have previously localized FA2 to this highly specific location, and predict that FAP16 will similarly localize (both FAP16 and FA2 are found in the flagellar proteome), possibly serving to dock the still-elusive calcium-sensing protein and/or the TRP15 channel. Indirect evidence suggests that the acid-stimulated influx of Ca2+ might not directly activate axonemal severing. Instead, the calcium influx might stimulate release of an internal store of Ca2+, which in turn triggers severing (reviewed in Quarmby 2009).

The current screen was not saturated: we isolated multiple new alleles of each of the three known deflagellation genes, but only two alleles of ADF3, single alleles for each of ADF2 and ADF4, and no new FA genes. This latter observation indicates that we still have not uncovered the microtubule-severing protein responsible for axonemal severing. It is also notable that we have yet to identify a calcium-binding protein to activate axonemal severing in response to the calcium signal. Intriguingly, only one of the new strains has a ts deflagellation defect (adf2-1), while another has a deflagellation defect that is conditional on nutrient status (adf4-1).

More genes remain to be discovered in the deflagellation pathway of Chlamydomonas, genes potentially also involved in the premitotic release of centrosomes. While our screen and subsequent mapping and complementation identified several interesting new genes to investigate, a future screen of ts lethal mutant strains could uncover important genes involved in both deflagellation and mitotic progression.

Acknowledgments

We are grateful to Professor Ryan Morin and his lab members Dan Fornika, Bruno Grande, and Selin Jessa for their invaluable assistance with sequencing and data analysis. Jeremy D. K. Parker, Kavisha Gunawardane, Adam Staniscia, Vy Tran, and Shinako Takimoto are thanked for their various contributions to the massive amount of genetic and molecular analysis behind the identification of ADF1. Mihajlo Todorovic contributed to the initial phase of the screen. This work was supported by a Natural Sciences and Engineering Research Council (NSERC) discovery grant to L.M.Q. An Accelerator Supplement award from NSERC to L.M.Q. allowed us to do the extensive sequencing required for this project.

Footnotes

  • Supplemental material is available online at www.g3journal.org/lookup/suppl/doi:10.1534/g3.116.034264/-/DC1

  • Communicating editor: S. L. Jaspersen

  • Received June 3, 2016.
  • Accepted August 9, 2016.
  • Copyright © 2016 Hilton et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Literature Cited

  1. ↵
    1. Anisimova M.,
    2. Gascuel O.
    , 2006 Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Syst. Biol. 55(4): 539–552.
    OpenUrlAbstract/FREE Full Text
  2. ↵
    1. Castresana J.
    , 2000 Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 17(4): 540–552.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Cerutti H.,
    2. Johnson A. M.,
    3. Gillham N. W.,
    4. Boynton J. E.
    , 1997 A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145(1): 97–110.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    1. Chen X.,
    2. Schulz-Trieglaff O.,
    3. Shaw R.,
    4. Barnes B.,
    5. Schlesinger F.,
    6. et al.
    , 2016 Manta: rapid detection of structural variants and indels for germline and cancer sequencing applications. Bioinformatics 32(8): 1220–1222.
    OpenUrlAbstract/FREE Full Text
  5. ↵
    1. Chevenet F.,
    2. Brun C.,
    3. Banuls A. L.,
    4. Jacq B.,
    5. Christen R.
    , 2006 TreeDyn: towards dynamic graphics and annotations for analyses of trees. BMC Bioinformatics 7: 439.
    OpenUrlCrossRefPubMed
  6. ↵
    1. Cingolani P.,
    2. Patel V. M.,
    3. Coon M.,
    4. Nguyen T.,
    5. Land S. J.,
    6. et al.
    , 2012a Using Drosophila melanogaster as a model for genotoxic chemical mutational studies with a new program, SnpSift. Front. Genet. 3: 35.
    OpenUrlCrossRefPubMed
  7. ↵
    1. Cingolani P.,
    2. Platts A.
    , L. Wang le, M. Coon, T. Nguyen et al., 2012b A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly (Austin) 6(2): 80–92.
    OpenUrlCrossRefPubMed
  8. ↵
    Craige, B., J. M. Brown, and G. B. Witman, 2013 Isolation of Chlamydomonas flagella. Curr. Protoc. Cell Biol. Chapter 3:Unit 3.41.1–3.41.9.
  9. ↵
    1. Cross F. R.,
    2. Umen J. G.
    , 2015 The Chlamydomonas cell cycle. Plant J. 82(3): 370–392.
    OpenUrlCrossRefPubMed
  10. ↵
    Dereeper, A., V. Guignon, G. Blanc, S. Audic, S. Buffet et al., 2008 Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 36(Web Server issue):W465–W469.
  11. ↵
    1. Edgar R. C.
    , 2004 MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5: 113.
    OpenUrlCrossRefPubMed
  12. ↵
    1. Engel B. D.,
    2. Ishikawa H.,
    3. Feldman J. L.,
    4. Wilson C. W.,
    5. Chuang P.-T.,
    6. et al.
    , 2011 A cell-based screen for inhibitors of flagella-driven motility in Chlamydomonas reveals a novel modulator of ciliary length and retrograde actin flow. Cytoskeleton (Hoboken) 68(3): 188–203.
    OpenUrlCrossRefPubMed
  13. ↵
    1. Finst R. J.,
    2. Kim P. J.,
    3. Quarmby L. M.
    , 1998 Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149(2): 927–936.
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Finst R. J.,
    2. Kim P. J.,
    3. Griffis E. R.,
    4. Quarmby L. M.
    , 2000 Fa1p is a 171 kDa protein essential for axonemal microtubule severing in Chlamydomonas. J. Cell Sci. 113(Pt 11): 1963–1971.
    OpenUrlAbstract/FREE Full Text
  15. ↵
    1. Guindon S.,
    2. Dufayard J. F.,
    3. Lefort V.,
    4. Anisimova M.,
    5. Hordijk W.,
    6. et al.
    , 2010 New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59(3): 307–321.
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Harris E. H.
    , 2009 The Chlamydomonas Sourcebook, Academic Press, Oxford, UK.
  17. ↵
    1. Hartzell L. B.,
    2. Hartzell H. C.,
    3. Quarmby L. M.
    , 1993 Mechanisms of flagellar excision. I. The role of intracellular acidification. Exp. Cell Res. 208(1): 148–153.
    OpenUrlCrossRefPubMedWeb of Science
  18. ↵
    1. Hilgendorf K. I.,
    2. Johnson C. T.,
    3. Jackson P. K.
    , 2016 The primary cilium as a cellular receiver: organizing ciliary GPCR signaling. Curr. Opin. Cell Biol. 39: 84–92.
    OpenUrlCrossRefPubMed
  19. ↵
    1. Ke Y. N.,
    2. Yang W. X.
    , 2014 Primary cilium: an elaborate structure that blocks cell division? Gene 547(2): 175–185.
    OpenUrlCrossRefPubMed
  20. ↵
    1. Kim S.,
    2. Tsiokas L.
    , 2011 Cilia and cell cycle re-entry: more than a coincidence. Cell Cycle 10(16): 2683–2690.
    OpenUrlCrossRefPubMedWeb of Science
  21. ↵
    1. Kim S. I.,
    2. Tai T. H.
    , 2011 Identification of genes necessary for wild-type levels of seed phytic acid in Arabidopsis thaliana using a reverse genetics approach. Mol. Genet. Genomics 286(2): 119–133.
    OpenUrlCrossRefPubMed
  22. ↵
    1. Kindle K. L.
    , 1990 High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 87(3): 1228–1232.
    OpenUrlAbstract/FREE Full Text
  23. ↵
    Kirschner, J. A., 2009 Mapping the deflagellation-defective adf1 gene in Chlamydomonas reinhardtii. M.Sc. Thesis, Simon Fraser University, Burnaby, BC.
  24. ↵
    1. Kumar S.,
    2. Stecher G.,
    3. Tamura K.
    , 2016 MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 33(7): 1870–1874.
  25. ↵
    Langmead, B., 2010 Aligning short sequencing reads with Bowtie. Curr Protoc Bioinformatics Chapter 11:Unit 11.7.
  26. ↵
    1. Li H.
    , 2011 A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing data. Bioinformatics 27(21): 2987–2993.
    OpenUrlAbstract/FREE Full Text
  27. ↵
    1. Li H.,
    2. Durbin R.
    , 2009 Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25(14): 1754–1760.
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Li H.,
    2. Handsaker B.,
    3. Wysoker A.,
    4. Fennell T.,
    5. Ruan J.,
    6. et al.
    , 2009 The Sequence Alignment/Map format and SAMtools. Bioinformatics 25(16): 2078–2079.
    OpenUrlAbstract/FREE Full Text
  29. ↵
    1. Lin H.,
    2. Dutcher S. K.
    , 2015 Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii. Methods Cell Biol. 127: 349–386.
    OpenUrlCrossRefPubMed
  30. ↵
    1. Lin H.,
    2. Miller M. L.,
    3. Granas D. M.,
    4. Dutcher S. K.
    , 2013 Whole genome sequencing identifies a deletion in protein phosphatase 2A that affects its stability and localization in Chlamydomonas reinhardtii. PLoS Genet. 9(9): e1003841.
    OpenUrlCrossRefPubMed
  31. ↵
    1. Lohret T. A.,
    2. McNally F. J.,
    3. Quarmby L. M.
    , 1998 A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation. Mol. Biol. Cell 9(5): 1195–1207.
    OpenUrlAbstract/FREE Full Text
  32. ↵
    1. Lyman K. A.,
    2. Chetkovich D. M.
    , 2015 Cortical compass: EML1 helps point the way in neuronal migration. Epilepsy Curr. 15(1): 43–44.
    OpenUrlCrossRefPubMed
  33. ↵
    1. Mahjoub M. R.,
    2. Montpetit B.,
    3. Zhao L.,
    4. Finst R. J.,
    5. Goh B.,
    6. et al.
    , 2002 The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J. Cell Sci. 115(Pt 8): 1759–1768.
    OpenUrlAbstract/FREE Full Text
  34. ↵
    1. Mahjoub M. R.,
    2. Rasi M. Q.,
    3. Quarmby L. M.
    , 2004 A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol. Biol. Cell 15(11): 5172–5186.
    OpenUrlAbstract/FREE Full Text
  35. ↵
    1. Marshall W. F.
    , 2008 Basal Bodies: platforms for building cilia. Curr. Top. Dev. Biol. 85: 1–22.
    OpenUrlCrossRefPubMedWeb of Science
  36. ↵
    1. McKenna A.,
    2. Hanna M.,
    3. Banks E.,
    4. Sivachenko A.,
    5. Cibulskis K.,
    6. et al.
    , 2010 The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20(9): 1297–1303.
    OpenUrlAbstract/FREE Full Text
  37. ↵
    1. Merchant S. S.,
    2. Prochnik S. E.,
    3. Vallon O.,
    4. Harris E. H.,
    5. Karpowicz S. J.,
    6. et al.
    , 2007 The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318(5848): 245–250.
    OpenUrlAbstract/FREE Full Text
  38. ↵
    1. Morgan L. C.,
    2. Birman C. S.
    , 2016 The impact of Primary Ciliary Dyskinesia on the upper respiratory tract. Paediatr. Respir. Rev. 18: 33–38.
    OpenUrlPubMed
  39. ↵
    1. Nachury M. V.
    , 2014 How do cilia organize signalling cascades? Philos. Trans. R. Soc. Lond. B. Biol. Sci. 369(1650) 20130465.
  40. ↵
    1. O’Toole E. T.,
    2. Dutcher S. K.
    , 2014 Site-specific basal body duplication in Chlamydomonas. Cytoskeleton (Hoboken) 71(2): 108–118.
    OpenUrlCrossRefPubMed
  41. ↵
    1. Parker J. D.,
    2. Bradley B. A.,
    3. Mooers A. O.,
    4. Quarmby L. M.
    , 2007 Phyl-ogenetic analysis of the Neks reveals early diversification of ciliary-cell cycle kinases. PLoS One 2(10): e1076.
    OpenUrlCrossRefPubMed
  42. ↵
    1. Parker J. D.,
    2. Hilton L. K.,
    3. Diener D. R.,
    4. Rasi M. Q.,
    5. Mahjoub M. R.,
    6. et al.
    , 2010 Centrioles are freed from cilia by severing prior to mitosis. Cytoskeleton (Hoboken) 67(7): 425–430.
    OpenUrlCrossRefPubMed
  43. ↵
    1. Pazour G. J.,
    2. Agrin N.,
    3. Leszyk J.,
    4. Witman G. B.
    , 2005 Proteomic analysis of a eukaryotic cilium. J. Cell Biol. 170(1): 103–113.
    OpenUrlAbstract/FREE Full Text
  44. ↵
    1. Peng G.,
    2. Shi X.,
    3. Kadowaki T.
    , 2015 Evolution of TRP channels inferred by their classification in diverse animal species. Mol. Phylogenet. Evol. 84: 145–157.
    OpenUrlCrossRefPubMed
  45. ↵
    1. Pollmann M.,
    2. Parwaresch R.,
    3. Adam-Klages S.,
    4. Kruse M. L.,
    5. Buck F.,
    6. et al.
    , 2006 Human EML4, a novel member of the EMAP family, is essential for microtubule formation. Exp. Cell Res. 312(17): 3241–3251.
    OpenUrlCrossRefPubMedWeb of Science
  46. ↵
    1. Quarmby L. M.
    , 1996 Ca2+ influx activated by low pH in Chlamydomonas. J. Gen. Physiol. 108(4): 351–361.
    OpenUrlAbstract/FREE Full Text
  47. ↵
    1. Quarmby L. M.
    , 2004 Cellular deflagellation. Int. Rev. Cytol. 233: 47–91.
    OpenUrlCrossRefPubMedWeb of Science
  48. ↵
    1. Quarmby L. M.
    , 2009 Deflagellation, pp. 43–69 in The Chlamydomonas Sourcebook Vol. 3, edited by G. Witman. Academic Press, Burlington.
  49. ↵
    1. Quarmby L. M.,
    2. Hartzell H. C.
    , 1994 Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii. J. Cell Biol. 124(5): 807–815.
    OpenUrlAbstract/FREE Full Text
  50. ↵
    1. Quarmby L. M.,
    2. Leroux M. R.
    , 2010 Sensorium: the original raison d’etre of the motile cilium? J. Mol. Cell Biol. 2(2): 65–67.
    OpenUrlAbstract/FREE Full Text
  51. ↵
    1. Quarmby L. M.,
    2. Parker J. D.
    , 2005 Cilia and the cell cycle? J. Cell Biol. 169(5): 707–710.
    OpenUrlAbstract/FREE Full Text
  52. ↵
    Rodriguez Pike, J. C., 2013 Discovery of a suppressor of ADF1 and the mapping of a new ADF gene, ADF2, in Chlamydomonas reinhardtii. M.Sc. Thesis, Simon Fraser University, Burnaby, BC.
  53. ↵
    1. Rymarquis L. A.,
    2. Handley J. M.,
    3. Thomas M.,
    4. Stern D. B.
    , 2005 Beyond complementation. Map-based cloning in Chlamydomonas reinhardtii. Plant Physiol. 137(2): 557–566.
    OpenUrlAbstract/FREE Full Text
  54. ↵
    1. Sarmah B.,
    2. Winfrey V. P.,
    3. Olson G. E.,
    4. Appel B.,
    5. Wente S. R.
    , 2007 A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proc. Natl. Acad. Sci. USA 104(50): 19843–19848.
    OpenUrlAbstract/FREE Full Text
  55. ↵
    1. Saunders C. T.,
    2. Wong W. S.,
    3. Swamy S.,
    4. Becq J.,
    5. Murray L. J.,
    6. et al.
    , 2012 Strelka: accurate somatic small-variant calling from sequenced tumor-normal sample pairs. Bioinformatics 28(14): 1811–1817.
    OpenUrlAbstract/FREE Full Text
  56. ↵
    1. Silflow C. D.,
    2. Kathir P.,
    3. Lefebvre P. A.
    , 1995 Molecular mapping of genes for flagellar proteins in Chlamydomonas. Methods Cell Biol. 47: 525–530.
    OpenUrlCrossRefPubMed
  57. ↵
    1. Sizova I.,
    2. Fuhrmann M.,
    3. Hegemann P.
    , 2001 A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 277(1–2): 221–229.
    OpenUrlCrossRefPubMedWeb of Science
  58. ↵
    1. Stirnimann C. U.,
    2. Petsalaki E.,
    3. Russell R. B.,
    4. Muller C. W.
    , 2010 WD40 proteins propel cellular networks. Trends Biochem. Sci. 35(10): 565–574.
    OpenUrlCrossRefPubMedWeb of Science
  59. ↵
    1. Wang J.,
    2. Barr M. M.
    , 2016 Ciliary Extracellular Vesicles: Txt Msg Organelles. Cell. Mol. Neurobiol. 36(3): 449–457.
    OpenUrlCrossRefPubMed
  60. ↵
    1. Wheeler G. L.,
    2. Joint I.,
    3. Brownlee C.
    , 2008 Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii. Plant J. 53(3): 401–413.
    OpenUrlPubMedWeb of Science
View Abstract
Previous ArticleNext Article
Back to top

PUBLICATION INFORMATION

Volume 6 Issue 10, October 2016

G3: Genes|Genomes|Genetics: 6 (10)

ARTICLE CLASSIFICATION

Mutant Screen Report
View this article with LENS
Email

Thank you for sharing this G3: Genes | Genomes | Genetics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel
(Your Name) has forwarded a page to you from G3: Genes | Genomes | Genetics
(Your Name) thought you would be interested in this article in G3: Genes | Genomes | Genetics.
Print
Alerts
Enter your email below to set up alert notifications for new article, or to manage your existing alerts.
SIGN UP OR SIGN IN WITH YOUR EMAIL
View PDF
Share

A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel

View ORCID ProfileLaura K. Hilton, Fabian Meili, Paul D. Buckoll, Julie C. Rodriguez-Pike, Courtney P. Choutka, Jaime A. Kirschner, Freda Warner, Mette Lethan, Fabian A. Garces, Jingnan Qi and View ORCID ProfileLynne M. Quarmby
G3: Genes, Genomes, Genetics October 1, 2016 vol. 6 no. 10 3409-3418; https://doi.org/10.1534/g3.116.034264
Laura K. Hilton
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Laura K. Hilton
Fabian Meili
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul D. Buckoll
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie C. Rodriguez-Pike
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Courtney P. Choutka
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaime A. Kirschner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Freda Warner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mette Lethan
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabian A. Garces
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingnan Qi
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lynne M. Quarmby
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lynne M. Quarmby
  • For correspondence: quarmby@sfu.ca
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation

A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel

View ORCID ProfileLaura K. Hilton, Fabian Meili, Paul D. Buckoll, Julie C. Rodriguez-Pike, Courtney P. Choutka, Jaime A. Kirschner, Freda Warner, Mette Lethan, Fabian A. Garces, Jingnan Qi and View ORCID ProfileLynne M. Quarmby
G3: Genes, Genomes, Genetics October 1, 2016 vol. 6 no. 10 3409-3418; https://doi.org/10.1534/g3.116.034264
Laura K. Hilton
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Laura K. Hilton
Fabian Meili
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul D. Buckoll
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie C. Rodriguez-Pike
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Courtney P. Choutka
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaime A. Kirschner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Freda Warner
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mette Lethan
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabian A. Garces
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingnan Qi
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lynne M. Quarmby
Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lynne M. Quarmby
  • For correspondence: quarmby@sfu.ca

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Related Articles

Cited By

More in this TOC Section

  • A Genetic Screen for Genes That Impact Peroxisomes in Drosophila Identifies Candidate Genes for Human Disease
  • An sRNA Screen for Reversal of Quinolone Resistance in Escherichia coli
  • A Genetic Screen in Drosophila To Identify Novel Regulation of Cell Growth by Phosphoinositide Signaling
Show more Mutant Screen Report
  • Top
  • Article
    • Abstract
    • Materials and Methods
    • Results and Discussion
    • Acknowledgments
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Supplemental
  • Info & Metrics

GSA

The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.

Online ISSN: 2160-1836

  • For Authors
  • For Reviewers
  • For Advertisers
  • Submit a Manuscript
  • Editorial Board
  • Press Releases

SPPA Logo

GET CONNECTED

RSS  Subscribe with RSS.

email  Subscribe via email. Sign up to receive alert notifications of new articles.

  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus

Copyright © 2019 by the Genetics Society of America

  • About G3
  • Terms of use
  • Permissions
  • Contact us
  • International access