Supporting Information for Katic, Xu, and Ciosk, 2015

Files in this Data Supplement:

• Supporting Information - Tables S1-S4 and Figures S1-S3 (PDF, 3 MB)
• Table S1 - Summary of comparisons of repair by sense and antisense oligonucleotides from this study and Ward (2015). (PDF, 69 KB)
• Table S2 - sgRNAs with complementarity of >20 nt to their target site can guide Cas9. (PDF, 97 KB)
• Table S3 - Mutagenicity of four sgRNAs targeting unc-22 correlate with predictions of the web tool based on the study of Doench et al. (2014). (PDF, 83 KB)
• Table S4 - sgRNAs with published efficiency in C. elegans and their scores according to the Doench et al. (2014) algorithm. (PDF, 131 KB)
• Figure S1 - Knock-in of a plasmid into a genomic locus upon non-homologous end joining-mediated repair of a Cas9/CRISPR lesion. (PDF, 2 MB)
• Figure S2 - Sequence of the 20^th exon of the unc-22 locus in a) N2 and b) in the unc-22(bch26) allele. (PDF, 82 KB)
• Figure S3 - Sequence of the lin-41 locus in a) N2 (partial) and b) in the lin-41(bch28) allele (partial). (PDF, 88 KB)